摘要
目的研究原花青素(PC)对动脉粥样硬化(AS)动物脂代谢、血总胆汁酸(TBA)、卵磷脂胆固醇酰基转移酶(LCAT)及病理组织学影响。方法按《保健食品检验与评价技术规范》取成年雄性长爪沙鼠经基础饲料适应性喂养1周后,建AS动物模型,按体质量分为基础对照组、模型对照组、低剂量组(25 mg/kg PC)、中剂量组(100 mg/kg PC)、高剂量组(150 mg/kg PC)、阳性对照组(非诺倍特80 mg/kg)6组,基础对照组饲以普通基础饲料喂养,其余各组均饲以高脂饲料喂养,分笼饲养,自由进食、饮水,每天灌胃1次,连续灌胃4周;1周后,模型对照组、低剂量组、中剂量组、高剂量组、阳性对照组均按700 000 U/kg VD3剂量灌胃3 d。实验期末,经股动脉采血,分离血清备用;采用全自动生化仪测TC、TG、HDL-C、LDL-C、TBA;并计算AI;循环酶法试剂盒测粪TBA排出量;步夹心ELISA测LCAT活性与Ox-LDL,解剖处死长爪沙鼠,留肝脏、主动脉进行病理组织学分析。结果与模型对照组相比,各剂量组TC、TG、LDL-C、血TBA、Ox-LDL均下降,LCAT活性、粪TBA排出量均上升,除低剂量组外,其余各组AI与模型对照组比均降低并有差异;与模型对照组比,各剂量组及阳性对照组各时间段体质量无差异;各剂量组及阳性对照组肝体比指数均降低并有差异;PC具有明显的拮抗高脂饮食对长爪沙鼠肝脏的损伤,进而减轻肝细胞的肿胀、变性等等病变程度的作用。结论 PC能调节AS脂代谢、TBA及LCAT活性,改善主动脉内膜和中膜钙化、脂变程度,并存在剂量反应关系。
Objective To explore the effect of proanthocyanidins（ PC） on atherosclerotic animal＇s lipid metabolism,pathological histology,the activity of TBA and LCAT. Methods The adult male gerbil（ Meriones unguiculatus） was fed with basal diet for1 w according to Technical Specifications for Testing and Evaluation of Health Food. Animal models of AS were established and divided into basic control group,model control group,low dose group（ 25 mg/kg PC）,middle dose group（ 100 mg/kg PC）,high dose group（ 150 mg/kg PC）,positive control group（ fenofibrate 80 mg/kg）. The basal control group was fed with normal basal diet,and the rest groups were fed with high-fat diet,fed in cage,fed freely,and fed with water once daily for 4 weeks. After1 w,the model control group,the low dose group,middle dose group,high dose group and positive control group were fed with700000 UVD3 for 3 days. At the end of the experiment period,blood was collected via the femoral artery and the serum was separated for use. Total cholesterol（ TC）,glycerol triglyceride（ TG）,high density lipoprotein cholesterol（ HDL-C）,low density lipoprotein cholesterol（ LDL-C） and total bile acid（ TBA） were detected with automatic biochemical analyzer. Atherogenic index（ AI） was counted,TBA excretion of feces was measured by a circulating enzyme kit. Double-antibody sandwich enzyme-linked immunosorbent assay（ ELISA） was used to detect LCAT activity and oxidative modification of low density lipoprotein（ Ox-LDL）. The gerbil was dissected and the liver was left and the aorta was analyzed by histopathology. Results Compared with model control group,the levels of TC,TG,LDL-C,Ox-LDL and the level of serum TBA reduced significantly,LCAT activity and the fecal TBA increased dramatically,AI was reduced significantly except low dose group. Compared with model control group,the difference of weight with other groups was not statistically significant. The index of liver/body ratio of other groups was reduced significantly. Histopath
出处
《中国卫生检验杂志》
CAS
2017年第24期3537-3540,共4页
Chinese Journal of Health Laboratory Technology
基金
浙江省科技厅公益性技术应用研究项目(2012C3-7096)
浙江省卫生厅医药卫生平台研究计划A类项目(骨干人才类)(2011RCA002)
浙江省中医药科学研究基金计划(2012ZB022)
