摘要
为了发掘菜用和观赏甘薯优异种质资源,通过对国家种质徐州甘薯试管苗库中1000余份资源材料进行鉴定,筛选了96份优异种质。利用30对SSR引物对入选材料进行了遗传多样性和群体结构分析,明确了这些材料遗传差异;并对入选材料12个表型质量性状进行主成分和聚类分析。结果表明:扩增的总条带数为275条,其中多态性条带为269条,多态率97.8%;利用DPS软件计算入选材料间的Nei72遗传距离为0.15~0.76,平均遗传距离0.66;群体结构分为3个组群,与分子标记聚类结果相似,表明入选材料有较大的遗传差异性;表型质量性状主成分分析得到5个主要成分,其累计贡献率达到80.50%;利用表型质量性状,可聚为8个组群。本研究通过分子标记与表型质量性状分析为下一步杂交选育菜用和观赏甘薯新品种提供了亲本选择信息。
In order to explore vegetable and ornamental sweetpotato germplasm resources, we identified more than 1000 accessions in Xuzhou national sweetpotato genebank, and selected 96 outstanding germplasm. 30 pairs of SSR primers were used to analyze genetic diversity and population structure, and genetic differences information of these selected materials was clearly got. 12 phenotypic quality traits data from all the accessions were also analyzed with principal components and clustering analysis. Results were as follows：the total number of amplified bands was 275 ,in which 269 with polymorphism,and polymorphic rate was 97.8% ;Nei72 genetic distance was between 0. 15 and 0.76 computed by DPS software with average distance 0. 66 ; population structure was divided into three groups, which was similar to the clustering results by molecular marker, indicating that those accessions had a greater genet- ic difference ;5 items,80.50% of cumulative contribution rate, were obtained from phenotypic quality traits principal components analysis;96 accessions could be clustered into eight groups by phenotypic quality traits. Molecular and phenotypic quality traits markers analysis results in this article provided a good reference for the parents selection information for new variety development of vegetable and ornamental sweetpotato.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2018年第1期57-64,共8页
Journal of Plant Genetic Resources
基金
国家自然科学基金(31461143017)
国家甘薯产业技术体系资源评价岗(CARS 2017-11-B-02)
国家农作物种质资源平台徐州甘薯子平台(NICGR-062)
江苏省重点研发项目(BE2016394)
江苏省"333"人才培养项目(BRA2016269)
关键词
菜用和观赏用甘薯
SSR标记
表型质量性状
遗传多样性
vegetable and ornamental sweetpotato
SSR marker
phenotypic quality traits
genetic diversity
