摘要
目的药用真菌经发酵后可获得多种次生代谢产物,这些代谢产物的含量远高于人工栽培的药用真菌。文中初步探讨发酵桦褐孔菌(FIO)对肝癌HepG-2细胞的凋亡作用。方法实验设置对照组(相同体积不含药物培养基)、UIO组(200 mg/L)、FIO组(200 mg/L)。硫酸苯酚法、Folin-Ciocalteu法、比色法测定未发酵、发酵桦褐孔多糖、萜类和多酚的含量;MTT法、流式细胞仪观察FIO对肝癌HepG-2细胞凋亡作用的影响。结果 FIO多糖含量[(10.140±0.849)mg/mL]较UIO中[(7.161±0.305)mg/mL]明显升高(P<0.01)。FIO三萜含量[(1.774±0.001)mg/mL]较UIO中[(1.358±0.004)mg/mL]明显升高(P<0.01)。FIO多酚[(9.979±0.022)mg/mL]较UIO[(6.314±0.237)mg/mL]明显升高(P<0.01)。Annexin V/PI双染结果显示,UIO组、FIO组均诱导肝癌HepG-2细胞凋亡。与对照组HepG-2细胞凋亡率(4.3%)比较,UIO组、FIO组(23.14%、27.37%)均升高(P<0.05),且FIO组较UIO组细胞凋亡率升高(P<0.05)。与对照组相比,UIO组、FIO组G0/G1期细胞比率显著升高(P<0.01),同时S期和G2/M期细胞数目所占总细胞数目比例均显著下降(P<0.01);与UIO组相比,G0/G1期细胞比率差异均有统计学意义(P<0.05)。结论 FIO相对于UIO可更好地诱导肝癌HepG-2细胞凋亡。
Objective A variety of secondary metabolites can be obtained after the fermentation of medicinal fungi. The content of these metabolites is much higher than that of cultivated medicinal fungi. We aimed to preliminarily study on the apoptosis of fermented Inonotus obliquus（FIO） on hepatocellular carcinoma HepG-2 cells.Methods The experiment was divided into 3 groups： the control group （The same volume of drug-free medium）, the UIO group （200 mg/L） and the FIO group （200 mg/L）. The contents of polysaccharides, terpenoids and polyphenols in unfermented Inonotus Obliquus （UIO） and FIO were determined by sulfuric acid phenol method, Folin-Ciocalteu method and colorimetric method. Effects of Fermented Inonotus obliquus on apoptosis of HepG-2 Cells were measured by MTT Method and Flow Cytometry.Results The contents of polysaccharides, terpenoids and polyphenols were significantly increased in FIO group ／[（10.140±0.849）mg/mL, （1.774±0.001）mg/mL, （9.979±0.022）mg/mL／] compared with thatin UIO group ／[（7.161±0.305）mg/mL, （1.358±0.004）mg/mL, （6.314±0.237）mg/mL／]（P〈0.01）. Both UIO and FIO group induced apoptosis of HepG-2 cells according to Annexin V/PI double staining. Compared with the control group （4.3%）, the apoptosis rate increased in UIO and FIO group （23.14%, 27.37%） （P〈0.05）. In addition, the apoptosis rate was higher in FIO group than that in UIO group （P〈0.05）. Compared with the control group, the ratio of G0/G1 phase cells was significantly increased and the ratio of S phase and G2/M phase was significantly decreased in UIO and FIO group （P〈0.01）. G0/G1 phase cell ratio differences were statistically significant compared with the UIO group （P〈0.05） Conclusion FIO can better induce the apoptosis of HepG-2 cells than UIO.
出处
《医学研究生学报》
CAS
北大核心
2018年第1期25-28,共4页
Journal of Medical Postgraduates
基金
吉林省科技发展计划项目(130303GX010211137)
吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2015]第8号)
