摘要
目的探讨发酵桦褐孔菌(FIO)诱导人肝癌细胞HepG-2凋亡的作用及机制。方法MTT法测定细胞增殖,HE染色法观察细胞形态,流式细胞仪检测细胞凋亡及细胞周期,蛋白免疫印迹法检测细胞内的凋亡相关蛋白的表达。结果FIO显著抑制HepG-2细胞的增殖,且具有时间和剂量相关性;FIO作用于HepG-2细胞后出现明显凋亡形态、凋亡率显著增加且呈浓度依赖性,细胞周期明显阻滞在G_(0)/G_(1)期;FIO各剂量组细胞内Caspase-3,Caspase-9,Cyt-c,Bax/Bcl-2和p53蛋白表达均显著升高,而ProCaspase-3和ProCaspase-9蛋白表达显著减少,且其作用呈现浓度依赖性。结论FIO对HepG-2细胞具有抑制增殖和诱导凋亡的作用,而线粒体通路可能参与调节FIO诱导HepG-2细胞凋亡的作用。
Objective To investigate the apoptotic effect of fermented Inonotus Obliquus(FIO) on human hepatocellular carcinoma HepG-2 cells.Methods MTT assay was used to determine the cell proliferation,and the cell morphology was observed by HE staining.Flow cytometry was used to determine the apoptosis and cell cycle of the cells,and the expressions of apoptosis-associated proteins in HepG-2 cells were detected by Western blotting.Results FIO significantly inhibited the proliferation of HepG-2 cells and which correlated with the time and the concentration of administration.After treat with FIO,HepG-2 cells were appeared the apoptotic morphology,the apoptosis rate of the cell was significantly increased in a concentration dependent manner and the cell cycle of the cell was arrested at G_(0)/G_(1) phase.The expressions of Caspase-3,Caspase-9,Cyt-c,Bax/Bcl-2 and p53 proteins were significantly increased and the expressions of ProCaspase-3 and ProCaspase-9 proteins were significantly decreased in the FIO groups.Conclusion FIO inhibits the proliferation and induces the apoptosis of HepG-2 cells,and mitochondrial pathway may participate in the FIO induced apoptosis of HepG-2 cells.
作者
朴淳健
魏宇轩
赵国钧
王虹力
陈正爱
PIAO Chun-jian;WEI Yu-xuan;ZHAO Guo-jun;WANG Hong-li;CHEN Zheng-ai(College of Medicine,Yanbian University,Yanji,Jilin 133002,China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2022年第12期2914-2918,共5页
Lishizhen Medicine and Materia Medica Research
基金
吉林省中医药科技项目(NO 2017119)
吉林省大学生创新创业训练计划项目(202110184061)。
关键词
发酵桦褐孔菌
肝癌HEPG-2细胞
细胞凋亡
Fermented inonotus obliquus
Hepatocellular carcinoma HepG-2 cells
Apoptosis
