摘要
本研究旨在克隆藏山羊FGF1基因的CDS序列,并获得其生物学特征,同时阐明其组织表达规律。选取2.5周岁健康公藏山羊6头,采集皮下脂肪、背最长肌、心脏、肝脏、脾脏、肺脏和肾脏组织样品,提取组织总RNA,利用RT-PCR方法克隆FGF1基因序列,同时利用实时定量PCR检测其m RNA在不同组织中的表达水平。结果表明,获得藏山羊FGF1基因序列1 165 bp(Gen Bank登陆号:KX509990),其中CDS为468 bp,5'UTR 255 bp和3'UTR 422 bp,编码155个氨基酸,藏山羊FGF1基因与山羊(KT899959)的核苷酸同源性为99.57%,氨基酸同源性为99.35%。FGF1 m RNA在藏山羊皮下脂肪组织中的表达水平较高,极显著高于其他组织(p<0.01)。本研究将为进一步研究藏山羊FGF1基因的结构和功能提供参考资料。
The aim of this study was to clone the CDS of fibroblast growth factor 1（FGF1）, analyze the sequence by bioinformatics, and clarify its expression characterization in different tissues. Six healthy, 2.5 years old male Tibetan goats were selecred for experiment animal. The tissue samples of subcutaneous fat, longissimus dorsi,heart, liver, spleen, lung and kidney were collected for the totle RNA extration. The Reverse Transcription PCR（RT-PCR） was used to clone Tibetan goat FGF1 gene sequence, the Real-time quantitative PCR（q PCR） was used to detect the expression level of FGF1 gene in different tissues. The results showed that the sequence of Tibetan goat FGF1 gene is 1 165 bp（Gen Bank accession number： KX509990）, containing 468 bp CDS, 255 bp 5’ UTR and422 bp 3’ UTR, encoding 155 amino acids. The nucleotide and amino acid sequence of Tibetan goat FGF1 share a similarity of 99.57% and 99.35% with those of goat（KT899959）. The m RNA expression level of FGF1 was high in subcutaneous fat, significantly higher than other tissues（p〈0.01）. These results will facilitate the research on the structure and function of FGF1 gene in Tibetan goat.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第12期5070-5076,共7页
Genomics and Applied Biology
基金
国家自然科学基金(31672395)
四川省"十三五"畜禽育种攻关项目(2016NYZ0045)共同资助
