摘要
通过PCR产物直接测序法对五华三黄鸡保种群120个个体进行线粒体DNA(mt DNA)D-loop序列测定,研究该品种的遗传多样性和系统进化。结果显示,五华三黄鸡保留着较高水平的遗传多样性。mt DNA D-loop的C+G含量(42.5%)低于T+A含量(57.5%),核苷酸多样性和核苷酸差异均数分别为0.01298±0.00051和6.750。共检测到33个突变位点,均为转换突变,包括23个T>C转换和10个A>G转换,占分析位点的6.35%。定义了32种单倍型,其中独享型单倍型19个,单倍型多样性为0.913±0.012,遗传距离为0.013±0.003。单倍型类群主要分布在进化枝A、B、C和E,其中B为优势单倍型类群。系统进化分析和进化枝地理分布特征表明,五华三黄鸡有4个母系来源,可能受北方家鸡南迁的影响。
For depicting genetic diversity and phylogeny of Wuhua Yellow chicken,one hundred and twenty samples were used to directly sequence using PCR products of mitochondrial DNA D-loop. The results showed that the Wuhua Yellow chicken obtained high levels of genetic diversity,with nucleotide diversity,average number of nucleotide differences and haplotype diversity for 0.01298 ± 0.00051,6.750 and 0.913 ± 0.012,respectively. The content of C+G(42.5%) was lower than T+A(57.5%). A total of 33 mutation sites were detected,including 23 T to C and 10 A to G transitions,accounted for 6.35% of the analyzed sequences. Thirty-two haplotypes weredefined,with 19 exclusive haplotypes and 13 shared haloptypes. The haplotype diversity and genetic distance were 0.913±0.012 and 0.013±0.003,respectively. These haplotypes were mainly distributed in clades A,B,C and E,and B was the dominated haplogroup. Phylogenetic analysis and geographical distribution indicated that there were four maternal origins to Wuhua Yellow chicken,which was influenced to some extent,by the gene flow of domestic chicken from northern China.
出处
《广东农业科学》
CAS
2017年第9期106-113,共8页
Guangdong Agricultural Sciences
基金
广东省自然科学基金(2014A030307018)
广东省公益研究与能力建设项目(2015A020208020
2016A030303068)
嘉应学院省市共建重点建设项目(嘉院[2017]27号)
