摘要
采用富集筛选法,以精氨酸为唯一氮源从羊草根际土壤中筛选出具有产多胺活力的菌株ccx5,探索产多胺细菌ccx5对植物生长的作用机制。以黄瓜为材料,采用营养液沙培的方法,研究了产多胺细菌ccx5对NaCl胁迫下的黄瓜幼苗生长的影响。结果表明:在NaCl的胁迫下,黄瓜幼苗的生长明显受到了抑制,且叶片中的叶绿素含量减少、丙二醛含量升高,抗氧化酶活性显著提高。而接种了产多胺菌ccx5的试验组的黄瓜幼苗的生长抑制得到缓解,叶片中的叶绿素含量和抗氧化酶活性明显高于NaCl处理组,但丙二醛含量有所降低。试验表明在NaCl胁迫下,产多胺细菌ccx5影响了植物的代谢过程,提高了黄瓜幼苗对盐胁迫的抗性。
We selected several bacterial strains with the capacity of producing polyamine around the rhizospheric soil of Chinese wildrye by using arginine as the sole nitrogen source through selectionenriching way,and explored the promoting mechanism of polyamine producing bacteria on plant growth. We used cucumber seedlings as materials and cultured them with nutritious solution in sands,and studied the effects of polyamine producing bacteria on the growth of cucumber seedlings treated with NaCl stress. The results indicated that NaCl stress significantly inhibited the growth of cucumber seedlings,meanwhile the content of chloroplast pigments reached a lower level,MAD reached a higher level,and the activity of antioxidase was on a significant rise,whereas the inhibition effect of cucumber seedlings ameliorated much when inoculated with polyamine producing bacteria,simultaneously the content of chloroplast pigments and activity of antioxidase were higher than the group treated with NaCl,with the exception of a lower content of MAD. The trial showed that polyamine producing bacteria can affect the metabolism of plant treated with NaCl and enhance plant resistance to NaCl stress.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2017年第6期675-682,共8页
Journal of Jilin Agricultural University
基金
国家自然科学基金项目(31300460)
吉林省大学生创业创新项目
关键词
黄瓜
产多胺细菌
NACL
盐胁迫
cucumber
polyamine producing bacteria
NaC1
salt stress
