摘要
根据微生物法降解甲醛(FD)的原理,以活性污泥中筛选的恶臭假单胞菌(Pseudomonas putida)W1为出发菌株,以甲醛作为碳源与能源,采用常压室温等离子体射流诱变系统(ARTP)进行诱变,结合高浓度甲醛梯度平板快速筛选,获得一高活性耐受甲醛突变株WMZ120-5,相对于原始菌株,突变株至少能耐受10 g/L的甲醛,最高浓度达到20 g/L,甲醛耐受浓度提高近10倍。当甲醛初始浓度≤3 g/L时,WMZ120-5在96 h内降解溶液中98%甲醛。经7次传代试验考察,突变株的遗传性状稳定。采用单因素逐一优化突变株降解甲醛条件,在pH 7.5,温度30℃和接种量5%的优化条件下,菌株在168 h可降解95%以上的4 g/L甲醛。突变株WMZ120-5对甲醛具有超高的耐受能力和良好的降解效率,在甲醛污染环境的微生物治理中具有应用潜力。
Formaldehyde（FD） has strong cytotoxicity to the organism and is seriously harmful to human health. So microbial degradation has become one of the most effective way for the removal of formaldehyde. The purpose of this study was to obtain mutants capable to metabolize high concentration of formaldehyde in existing strains. Atmospheric and room tempera-ture plasmas（ARTP） mutagenesis system can help to cause high mutation rate and wide mutational spectrum with lower damage. Taking the strain Pseudomonas putida W1 as the original strain,mutants were quickly obtained by using ARTP mutation and then compared them with gradient plate screening method. The results showed that mutant WMZ120-5 in the solid media culture could tolerate maximum concentrations of formaldehyde ranging from 10 g/L to 20 g/L, showing more than 10 times higher compared with those by the wild-type W1 under the same conditions. Formaldehyde removal efficiencies above98% in 96 h was obtained at formaldehyde concentration equal to or less than 3 g/L. Its character was stable after seven generatations. And optimal parameters for 4 g/L formaldehyde-degrading condition was initial pH 7.5, 30 ℃ and 5% inoculum dose. More than 95% formaldehyde was consumed within 168 h under optimized conditions. The results showed that mutant WMZ120-5 has a good tolerance to formaldehyde and high degradation efficiency to formaldehyde, and potential use for re-mediation of formaldehyde pollution.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2017年第11期95-102,共8页
Environmental Science & Technology
基金
中国博士后科学基金项目(2013M541735)
国家级与安徽省大学生创新创业训练计划项目(201510363074
20161036300209)
安徽工程大学预研项目(2016yyzr07)
关键词
恶臭假单胞菌
甲醛
降解特性
常压室温等离子体(ARTP)
梯度平板筛选法
Pseudomonas
formaldehyde
degradation characteristics
atmospheric and room temperature plasmas （ARTP）
gradient plate screening
