摘要
通过花粉管通道法将pCMBIA3300-Cry1Iem载体中的Cry1Iem基因转入大豆品种"吉农28"中,对经过抗草铵膦筛选的转基因植株进行PCR检测,初步确定阳性植株,对转基因阳性植株进行Southern blotting检测,结果发现有5株出现杂交信号,证明Cry1Iem基因整合到受体的基因组中。利用实时荧光定量PCR测定T1代阳性植株的Cry1Iem基因在mRNA水平上的表达量,证明Cry1Iem基因在受体材料中获得了表达。
By pollen-tube pathway method,p CMBIA3300-Cry1 Iem gene was transferred into JN28 soybean. Preliminary determination of positive plants was conducted by PCR detection of transgenic plants with glufosinate resistant screening. Detection of the transgenic plants was conducted by Southern blotting and hybridization signal appeared in 5 strains. The results show that Cry1 Iem gene integrated into the genome of receptor materials. Expression of Cry1 Iem gene at the mRNA level was measured by real-time fluorescence quantitative PCR. It was proved that the Cry1 Iem gene was expressed in the receptor material.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2017年第6期665-669,共5页
Journal of Jilin Agricultural University
基金
转基因生物新品种培育重大专项(2014ZX08004-004)
