摘要
针对花粉管通道法导入苦瓜DNA的籼稻子代D9和受体RT,采用IlluminaHiSeq^(TM)平台进行全基因组重测序,分析了精米的基本营养成分、总皂苷成分以及膨胀势和米饭的食味品质。结果表明:苦瓜的部分DNA片段被整合进了受体水稻的DNA中,使生理活性成分总皂苷含量显著提高;苦瓜基因对水稻的营养成分有改善作用,使脂肪含量增加,膳食纤维与灰分的含量有所减少。
Offspring D9 from indica rice imported bitter melon DNA by pollen tube channel method and the receptor(RT),and the whole genome was sequenced by Illumina HiSeq^(TM).Meanwhile,the nutritional composition contents and total saponin content in milled rice,and the swelling power and the edible quality of milled rice were analyzed.The results indicated that parts of the melon’s DNA fragment were integrated into the rice’s DNA and the total saponin content of physiological active ingredient was significantly increased.The bitter melon gene caused the improvement on the nutrients and make the fat content increase,dietary fiber and ash content to reduce.
作者
袁洁
何登骥
何加太
詹庆才
周昆
何艳
阳永建
魏颖娟
陶湘林
唐汉军
YUAN Jie;HE Dengji;HE Jiatai;ZHANG Qingcai;ZHOU Kun;HE Yan;YANG Yongjian;WEI Yingjuan;TAO Xianglin;TANG Hanjun(Longping Branch,Graduate School of Hunan University,Changsha,Hunan 410125,China;Rice Research Institute of Hunan Agricultural Sciences Academy,Changsha,Hunan 410125,China;Biotechnology Research Institute of Hunan Agricultural Sciences Academy,Changsha,Hunan 410125,China;Agricultural Products Processing Institute of Hunan Agricultural Sciences Academy,Changsha,Hunan 410125,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第4期378-384,共7页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省重点研发计划项目(2019NK2121)
湖南省农业科学院创新基金项目(2018ZD04–1–2)。
关键词
籼稻
苦瓜
花粉管通道法
碱基序列
营养品质
indica rice
bitter melon
pollen tube channel method
base sequence
nutritional quality
