摘要
目的:探讨血黏蛋白1(Mucin 1,MUC1)mRNA和细胞角蛋白19片段(cyfra21-1)对非小细胞肺癌(non-small cell lung cancer,NSCLC)的诊断价值。方法:选取80例NSCLC患者(NSCLC组)、50例肺部良性病变患者(良性组)以及40例健康体检者(健康组)为研究对象;检测各组受试人员血MUC1mRNA和cyfra21-1水平,分析MUC1 mRNA和cyfra21-1在NSCLC诊断中的应用价值,比较不同病理特征NSCLC患者血MUC1 mRNA和cyfra21-1阳性率差异。结果:NSCLC组血MUC1 mRNA和cyfra21-1蛋白水平明显高于良性组和健康组(P<0.05),而良性组和健康组血MUC1 mRNA和cyfra21-1水平差异无统计学意义(P>0.05)。MUC1 mRNA诊断NSCLC的最佳临界值为MUC1 mRNA>2.14,其诊断灵敏度为68.8%,特异度为94.4%;血清cyfra21-1最佳临界值为cyfra21-1>3.20 ng/mL,其灵敏度为75.0%,特异度为98.9%。MUC1 mRNA和cyfra21-1联合诊断的灵敏度为87.5%,显著高于MUC1mRNA和cyfra21-1单独检测(P<0.05),而特异度与两者单独检测差异无统计学意义(P>0.05)。血MUC1 mRNA和cyfra21-1表达阳性率与患者肿瘤大小、分化程度、TNM分期及淋巴结转移有关(P<0.05),而与患者年龄、性别以及组织类型无关(P>0.05)。结论:血MUC1 mRNA和cyfra21-1检测有助于NSCLC的临床诊断和病情评估。
Objective： To investigate the value of peripheral blood Mucin 1（MUC1） mRNA and cytokeratin 19 fragment（cyfra21-1） in diagnosis of non-small cell lung carcinoma（NSCLC）. Methods： Eighty patients with NSCLC（NSCLC group）, 50 patients with benign pulmonary disease（benign group） and 40 healthy cases（healthy group）were enrolled in this study. The levels of MUC1 mRNA and cyfra21-1 were detected. The values of MUC1 mRNA and cyfra21-1 in diagnosis of NSCLC were analyzed. The differences of MUC1 mRNA and cyfra21-1 positive ratewere compared in patients with different pathological features. Results： The levels of MUC1 mRNA and cyfra21-1 in NSCLC group were significantly higher than those in the benign group and the healthy group（P〈0.05）; while there was no significant difference in levels of MUC1 mRNA and cyfra21-1 between the benign group and the healthy group（P〈0.05）. The best cut-off value of MUC1 mRNA in diagnosis of NSCLC was MUC1 mRNA 2.14 with the sensitivity was 68.8% and specificity was 94.4%. The best cut-off value of cyfra21-1 was cyfra21-1 3.20 ng/mL with the sensitivity was 75.0% and specificity was 98.9%. The sensitivity of joint application of MUC1 mRNA and cyfra21-1 was 87.5%, which was significantly higher than that of MUC1 mRNA or cyfra21-1 alone（P〈0.05）, while there was no significant difference in specificity（P〈0.05）. The positive rates of MUC1 mRNA and cyfra21-1 were significantly correlated with tumor size, differentiated degree, TNM staging, and lymph node metastasis（P〈0.05）,but no correlated with age, gender and histologic type（P〈0.05）. Conclusion： The detections of MUC1 mRNA and cyfra21-1 are helpful in clinical diagnosis and disease evaluation of NSCLC.
出处
《临床与病理杂志》
2017年第11期2395-2401,共7页
Journal of Clinical and Pathological Research
基金
国家自然科学基金(81472179)
上海市加强公共卫生体系建设3年行动计划(2015~2017年)(15GWZK0301)~~
