摘要
目的:探讨非小细胞肺癌患者外周血中生存素和血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)的表达及临床意义。方法:应用荧光定量PCR法检测42例非小细胞肺癌(non-small cell lung cancer,NSCLC)患者及20例肺部良性疾病患者外周血中生存素mRNA和VEGF mRNA的表达。结果:NSCLC患者外周血中生存素mRNA和VEGF mRNA的表达水平明显高于肺部良性疾病患者(P=0.006;P=0.001)。NSCLC患者外周血中生存素mRNA的阳性表达率为59.5%,VEGF mRNA的阳性表达率为57.1%,两者联合检测阳性率可提高至76.2%。肺部良性疾病患者生存素mRNA阳性表达率为5%,VEGF mRNA均为阴性,明显低于NSCLC患者(P均<0.001)。NSCLC患者外周血中生存素mRNA和VEGF mRNA的表达与肿瘤的TNM分期显著相关(P<0.001;P=0.008),而与其他临床特征无明显相关性。结论:外周血生存素mRNA和VEGF mRNA表达可作为肺癌细胞的分子标志物用于临床诊断,两者联合检测可提高NSCLC患者外周血癌细胞的检出率。
Objective： To evaluate the clinical significance of mRNA expression of survivin and vascular endothelial growth factor（VEGF） in the peripheral blood from patients with non-small cell lung cancer（NSCLC）.Methods： Real-time fluorescence quantitation polymerase chain reaction（RT-PCR） was used to detect mRNA expression of survivin and VEGF in peripheral blood samples from 42 patients with NSCLC and 20 patients with benign lung disease.Results： The mRNA expression of survivin and VEGF in NSCLC patients were significantly higher than those in benign lung disease patients（P=0.006;P=0.001,respectively）.The positive rates of mRNA expression of survivin and VEGF in peripheral blood from patients with NSCLC were 59.5 %（25/42） and 57.1%（24/42）,respectively.Combined with the two mRNA expression made the positive rate raise to 76.2%,while the positive rates of mRNA expression of survivin and VEGF in peripheral blood from patients with benign lung disease were 5%（1/20） and 0%,respectively,which were markedly lower than those of patients with NSCLC（both P0.001）.For patients with NSCLC,the tumor TNM stage was significantly correlated with mRNA expression of survivin and VEGF（P0.001;P=0.008,respectively）,whereas there were no correlation between mRNA expression of survivin and VEGF and other pathologic characteristics.Conclusion： The mRNA expression of survivin and VEGF in peripheral blood could be used as the molecular markers for diagnosis of NSCLC;Combined with the two mRNA expression in peripheral blood could raise the detection rate of cancer cell in peripheral blood.
出处
《江苏大学学报（医学版）》
CAS
2013年第1期34-38,共5页
Journal of Jiangsu University：Medicine Edition
关键词
荧光定量PCR
非小细胞肺癌
生存素
血管内皮细胞生长因子
fluorescence quantitation PCR
non-small cell lung cancer
survivin
vascular endothelial growth factor
