摘要
为研究磁感应热疗联合艾迪注射液对人淋巴瘤Daudi细胞的作用,首先进行磁性介质对Daudi细胞生物相容性的研究以及磁性介质升温性能的检测,运用CCK-8法测出不同艾迪药物质量浓度对细胞增殖率的影响选出本实验工作浓度,将对数生长期的人淋巴瘤细胞Daudi分别暴露于磁场、艾迪注射液、磁场与艾迪联合作用下,采用流式细胞技术分析各组实验作用下对细胞凋亡、细胞周期的影响。结果表明:本实验选用的不锈钢空心球具有良好的发热效率以及细胞相容性;本研究选用75 mg/m L作为工作浓度;磁感应热疗作用Daudi细胞后,细胞存活率为(78.48±0.95)%,联合艾迪注射液治疗后,细胞存活率为(9.25±2.05)%(P<0.01);磁感应热疗联合艾迪注射液能够增加细胞凋亡率,而对细胞周期改变不明显。由此可知,磁感应热疗磁性介质应用于淋巴瘤治疗在细胞水平是可行的;磁感应热疗单独使用可以诱导Daudi细胞凋亡,艾迪注射液与其联合应用时能表现出协同抗淋巴瘤细胞作用。
To investigate the effects of magnetic-mediated hyperthermia（MMH） in combination with Aidi injection on human lymphoma Daudi cells in vitro, the biocompatibility and temperature rising behavior of selected magnetic media are studied . Then cell proliferation in culture is studied in human lymphoma Daudi cell fines exposed to MMH and Aidi separately and in combination. The cell apoptosis and cycle of Daudi cells are analyzed by flow cytometry. Theoretical calculations indicate that the biocompatibility of MMH magnetic media is satisffactory. In vivo experiments it also generates heat effectively. The survival rate of Daudi cells exposed to MMH （300 kHz, 40 Gs, 30 min） is （78.48＋0.95）%, but significantly decreases to （9.25＋2.05）% （P〈0.01） when being exposed to the combination of MMH and Aidi. MMH in combination with Aidi can promote Daudi cells apoptosis, but the change in cell cycle is not obvious. The MMH magnetic media is feasible to Daudi cells in vitro. MMH alone could induce apoptosis of Daudi cells and significantly enhance anti-tumor effect with Aidi injection.
出处
《科技导报》
CAS
CSCD
北大核心
2017年第23期52-57,共6页
Science & Technology Review
基金
国家自然科学基金项目(81570188
81470308)
