摘要
以桦褐孔菌多糖IOP3a为原料,采用乙酸酐法制备乙酰化桦褐孔菌多糖(Ac-IOP3a),以乙酰化取代度为指标,考察乙酸酐用量、反应温度和反应时间对取代度的影响。在单因素试验的基础上,采用响应面法对IOP3a乙酰化工艺进行优化,并通过Ac-IOP3a对·OH、DPPH·和O_2^-·的清除作用探讨其抗氧化活性。结果表明,桦褐孔菌多糖IOP3a乙酰化最佳工艺为:乙酸酐用量3.3 mL(固定IOP3a多糖量100 mg),反应时间3.1 h,反应温度64℃,在此条件下,Ac-IOP3a的取代度为0.416。抗氧化研究表明:与IOP3a多糖相比,Ac-IOP3a多糖的抗氧化活性明显增强。
A polysaccharide IOP3a from Inonotus obliquus was used as raw materials. IOP3a was acetylated by acetic anhydride and its acetylated modification was studied. Effects of acetic anhydride dosage, reaction temperature and reaction time on the substitution degree were investigated through the single factor experiment. The optimization of acetylation of IOP3a was optimized by response surface experiments. Antioxidant activity of Ac-IOP3a in vitro was assessed by DPPH, hydroxyl and superoxide anion free radical scavenging assays. The optimal conditions were acetic anhydride amount of 3.3 mL（IOP3a 100 mg）, reaction time of 3.1 h and reaction temperature of 64 ℃. Under this condition, the degree of substitution was 0.416. Compared with IOP3a, antioxidant activity of Ac-IOP3a enhanced significantly.
出处
《食品工业》
北大核心
2017年第12期184-189,共6页
The Food Industry
基金
国家星火计划项目(2015GA690021)
关键词
桦褐孔菌多糖
乙酰化
响应面
抗氧化
polysaccharides from Inonotus obliquus
ablation
response surface methodology
antioxidant activity
