摘要
为确定桦褐孔菌发酵液提取物的毒性级别,研究对H22肝癌小鼠的抗肿瘤及免疫调节作用。依据2004年实施的《急性毒性试验国家标准》进行急性毒性研究和级别鉴定,同时建立H22肝癌的荷瘤小鼠模型进行小鼠体内抗肿瘤的研究,并采用MTT法检测荷瘤小鼠自然杀伤细胞活性(NK)及T淋巴细胞增殖情况。结果表明,桦褐孔菌发酵液提取物(IOFE)的LD50为15.02 g·kg-1>15 g·kg-1,属无毒级别。与阴性对照和阳性对照比,体重、采食量均稳定增长,抑瘤率为44.25%,可显著提高NK细胞活性67.13%(p<0.01),但对T淋巴细胞增殖率效果不明显。桦褐孔菌发酵液提取物具有较好的抗肿瘤作用,且毒性极低,抗肿瘤作用可能是通过提高机体免疫活性尤其是NK细胞的杀伤活性实现的。
In this work,the toxicity level of the extract from fermention liquid of Inonotus obliquus（IOFE） was tested and the inhibition of hepatoma H22 and immunomodulatory effects in vivo were assessed.According to the acute toxicity tests national standard implemented in 2004,the toxicity level were tested and analyzed.Meanwhile,the models of hepatoma H22 established in mouse were used to study the antiumor effect of IOFE.Then the nature killer（NK） cell acticity and T lymphocyte proliferation rate were detected by MTT method.The results showed that IOFE was no toxicities because LD50 was 15.02 g·kg-115.00 g·kg-1.Compared with positive and nagative controls,the body weight and the food intake of mice treated by IOFE increased steadily and the nature killer（NK） cells activity was improved significantly which was 67.13%（p0.01）.Also the inhibition of hepatoma H22 was 44.25%.While the effect on T lymphocyte proliferation rate was not obvious.In conclusion,the tumor can be inhibited well in vivo by IOFE,and the reason may be that it can imporve the body’s immunity,especially enhance NK cell acticity.
出处
《中国食用菌》
北大核心
2010年第3期37-39,共3页
Edible Fungi of China
基金
国家科技攻关项目
生物多糖关键技术及应用研究(2001BA507A18)
山东省科技攻关项目(2007GG2009017)资助
关键词
半数致死量
抑瘤率
自然杀伤细胞活性
淋巴细胞增值率
Median lethal dose
Inhibitory rate
Nature killer cell activity
Lymphocyte proliferation
