摘要
为进一步探讨植物多糖结构与活性之间的关系，采用乙酸酐为乙酰化试剂，以大粒车前子多糖（PLP）为原料，制得乙酰化修饰大粒车前子多糖（Ac—PLP）。考察乙酸酐剂量、反应温度、反应时间对此乙酰化修饰反应的影响，并通过正交试验确定最适反应条件为乙酸酐体积分数10％、反应温度30℃、反应时间2．5h。进一步在体外树突状细胞模型中研究发现，当取代度在0．06～0．1范围内时，乙酰化修饰大粒车前子多糖促进树突状细胞分泌细胞因子IL-12p70的能力较强，且在取代度0．08左右时达到最大。本研究结果提示对大粒车前子多糖进行乙酰化修饰可作为其改性方向，为大粒车前子多糖功能性产品开发提供了相应的理论依据。
Acetylatr..d polysaccharide from the seeds of Plantago asiatica L. （Ac-PLP） which provides materials for studying the structure-activity relationship of plant polysaccharides was prepared by using acetic anhydride as acetylating agent. Factors influencing the modification reaction, such as acetic anhydride dosage, temperature and reaction time, were investigated. The optimal reaction conditions were determined using orthogonal array design as 10% of acetic anhydride, 30 and 2.5 h. Further in vitro investigation demonstrated that Ac-PLPs with a substitution degree from 0.06 to 0.1 promoted IL-12p70 secretion by dendritic cells and the strongest effect was observed when the degree of substitution was about 0.08. The results in the current study reveal that acetylation can be an alternative way to modify PLPs and provide a theoretic basis for the development of functional products with PLPs.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第22期1-4,共4页
Food Science
基金
国家自然科学基金重点项目(31130041)
国家自然科学基金地区科学基金项目(21062012
31260364)
"十二五"国家科技支撑计划项目(2012BAD33B06)
江西省自然科学基金青年科学基金项目(2010GQY0245)
江西省教育厅青年科学基金项目(GJJ11050)
关键词
大粒车前子
多糖
乙酰化
树突状细胞
seeds of Plantago asiatica L.
polysaccharide
acetylation
dendritic cells
