摘要
在序批式(sequencing batch reactor,SBR)反应器中,通过分段厌氧-好氧(厌氧后排水)运行方式,在以葡萄糖为碳源、P/C比小于2/100的条件下,成功实现了聚糖菌(glycogen accumulating organisms,GAOs)的驯化富集,厌氧段磷酸盐的释放量(phosphorus release amounts,PRA)稳定在1.0 mg·L-1以内,胞内糖原(glycogen,gly)含量是初始阶段的1.2倍。驯化后的GAOs分别以NO_2^--N、NO_3^--N为电子受体经厌氧-缺氧运行方式,可进行内源反硝化反应过程。GAOs在内源反硝化过程中依次利用胞内的聚β-羟基戊酸酯(poly-β-hydroxyvalerate,PHV)、聚β-羟基丁酸酯(poly-β-hydroxyvalerate,PHB)和gly作为内碳源。在22℃时,反硝化聚糖菌(denitrifying glycogen accumulating organisms,DGAOs)以NO_2^--N、NO_3^--N为电子受体平均比内源反硝化速率分别为0.067 g N·(g VSS)-1·d-1、0.023 g N·(g VSS)-1·d-1,常温短程内源反硝化速率约是全程内源反硝化速率的3倍。
Glycogen accumulating organisms（GAOs） has been enriched in the anaerobic-aerobic sequencing batch reactor（AO-SBR）（drained after anaerobic） with glucose as the carbon source and the P/C ratio less than 2/100. PRA was less than 1.0 mg·L-1. The content of gly was 1.2 times the initial stage of GAOs enrichment. The domesticated GAOs cultured in anaerobic-anoxic operation could proceed endogenous denitrification reaction with NO2--N and NO3--N as endogenous carbon source. GAOs used intracellular poly-β-hydroxyvalerate（PHV）, poly-β-hydroxyvalerate（PHB） and glycogen（gly） in turns as carbon source in endogenous denitrification process. The average endogenous denitrification rate of DGAOs using NO2--N and NO3--N as electron acceptors were 0.067 g N·（g VSS）-1·d-1 and 0.023 g N·（g VSS）-1·d-1 at 22℃, respectively. Short-range endogenous denitrification rate was about three times as much as endogenous denitrification rate at normal temperature.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2017年第12期4731-4738,共8页
CIESC Journal
基金
国家自然科学基金项目(51578014)
北京市教委资助项目~~
关键词
聚糖菌
厌氧
需氧
曝气
富集
内源反硝化
glycogen accumulating organisms （GAOs）
anaerobic
aerobic
aeration
enrichment
endogenous denitrification
