摘要
采用序批式生物反应器(SBR),以厌氧-好氧-缺氧的运行方式,研究了低C/N比下内碳源驱动的短程硝化反硝化工艺运行性能.结果表明,反应器内可同时富集反硝化聚糖菌(DGAOs)和氨氧化细菌(AOB).DGAOs可以利用聚-β-羟基脂肪酸酯(PHA)为内碳源进行反硝化,且利用的PHA中PHB(聚-β-羟基丁酸酯)占主要部分.稳定运行后,第39d厌氧末期污泥胞内存储物质在荧光显微镜下清晰可见,内碳源存储的PHA在缺氧阶段净消耗量为2.34mmol C/L,较文献报道值高29%.经过55d的驯化后,SBR系统达到了较为稳定的脱氮效果,平均氨氮去除率为(93.13%±4.91%),内碳源反硝化效率为(49.62%±8.97%).驯化后的污泥淘汰了反硝化聚磷菌(DPAOs)和亚硝酸盐氧化菌(NOB),富集了DGAOs和AOB,其丰度从接种时的0.13%和0.20%分别上升到7.13%和1.11%,实现了低C/N下内碳源驱动短程硝化反硝化.
The operation performance of short-cut nitrification and denitrification process driven by internal carbon source at low C/N ratio was studied using Sequencing Batch Reactor(SBR) and anaerobic-aerobic-anoxia operation mode. The results showed that denitrifying golyphosphate accumulating Organisms(DGAOs) and ammonia-oxidizing Bacteria(AOB) can be enriched simultaneously in the reactor. DGAOs can use poly-β-hydroxyalkanoate(PHA) as internal carbon source for denitrification, and PHB(poly-β-hydroxy butyrate) was the main part of PHA. After stable operation, intracellular substances stored in sludge at the end of anaerobic phase could be clearly seen under fluorescence microscope at the 39 th day, and 2.34 mmol C/L in PHA stored by internal carbon source was consumed, which was 29% higher than the value reported in literature. After 55 days of domestication, the SBR system achieved a relatively stable denitrification effect. The average ammonia nitrogen removal rate was 93.13%±4.91%, and the denitrification efficiency of internal carbon source was 49.62%±8.97%. After domestication, denitrifying phosphate accumulating organisms(DPAOs) and nitrite oxidizing bacteria(AOB) were eliminated, denitrifying glycan bacteria and ammonia-oxidizing bacteria were enriched, and their abundance increased from 0.13% and 0.20% at inoculation to 7.13% and 1.11%, respectively,realizing the short-cut nitrification and denitrification driven by internal carbon source at low C/N.
作者
周倩
张林
唐溪
唐崇俭
ZHOU Qian;ZHANG Lin;TANG Xi;TANG Chong-jian(Chinese National Engineering Research Center for Control and Treatment of Heavy Metal Pollution,School of Metallurgy and Environment,Central South University,Changsha 410083,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2021年第12期5673-5679,共7页
China Environmental Science
基金
国家自然科学基金资助项目(51878662)
湖南省科技计划重大项目(2017SK2420)。
关键词
反硝化聚糖菌
氨氧化细菌
SBR
短程硝化
内碳源反硝化
denitrifying golyphosphate accumulating orgnisms
ammonia oxidizing bacteria
SBR
short-cut nitrification
internal carbon source denitrification
