摘要
目的观察针灸联合温脾补肾方对急性溃疡性结肠炎大鼠血管活性肠肽、血清肿瘤坏死因子-α(TNF-α)和白细胞介素水平的影响并探讨其机制。方法采用免疫学方法并加局部刺激建立溃疡性结肠炎大鼠模型,随机分为模型组、针灸组、温脾补肾方组和针灸联合温脾补肾方组,针灸组选取天枢和足三里穴针灸,温脾补肾方组给予灌胃,联合组给予针灸和灌胃,2周后HE染色观察结肠组织病理改变,比较两组患者的血管活性肠肽(VIP)、血清TNF-α和白细胞介素-6(IL-6)炎症因子,超氧化物歧化酶(SOD)、脂质过氧化物(LPO)、谷胱甘肽过氧化物酶(GSH-PX)氧化应激水平。结果模型组大鼠结肠黏膜充血、水肿,皱襞减少,可见溃疡,肠管增粗,肠壁变薄,大量炎症细胞浸润,外壁与外周组织黏连;针灸组和温脾补肾方组结肠黏膜充血、水肿减轻,溃疡减轻,肠壁变厚,炎症细胞减少,少量组织黏连;联合组未见结肠黏膜充血、水肿、溃疡和组织黏连,少量炎细胞浸润。试验组VIP、TNF-α及IL-6水平均明显低于模型组,且联合组优于针灸组和温脾补肾方组(P<0.05)。试验组SOD和GSH-PX水平明显高于模型组,而LPO水平明显低于模型组,模型组,且联合组优于针灸组和温脾补肾方组(P<0.05)。结论针灸联合温脾补肾方治疗急性溃疡性结肠炎大鼠可改善结肠黏膜,降低炎症反应,改善氧化应激指标。
Objective： To investigate the effect of acupuncture combined with Wenpi Bushen Decoction on va- soactive intestinal peptide,serum tumor necrosis factor-or and interleukin in acute ulcerative colitis rats. Meth- ods： The ulcerative colitis rat models were established by immunological method and local stimulation,and were randomly divided into the model group,the acupuncture group,Wenpi Bushen Decoction group and acupuncture combined with Wenpi Bushen Decoction group. The acupuncture group was given Tianfu and Zusanli point acupuncture,and Wenpi Bushen Decoction group was given by gastric. The combination group was given acupuncture and lavage. After two weeks,the colon tissue pathological changes were observed,and the vasoactive intestinal peptide （TNF-α）, interleukin-6 （IL -6） inflammatory factors, superoxide dismutase （SOD）, lipid perox- ides （LPO）,glutathione Peroxidase （GSH-PX） oxidative stress levels were compared between the two groups. Re- suits： In the model group,colonic mucosa congested;edema and plica reduced. The ulcer was seen,the intestinal tract thickened, the intestinal wall thinned, a large number of inflammatory ceils infiltrated, and the outer wall ad- hered to the peripheral tissue. In the acupuncture group and Wettpi Bushen Decoction group,colonic mucosa con- gested and edema reduced. The ulcer relieved and bowel wall thickened;the inflammatory cells were reduced and a small amount of tissue adhered. In the combination group,there was no colonic mucosa hyperemia,edema,ulcer and tissue adhesion,with a small number of inflammatory cells infiltrations. Tile levels of VIP,TNF-α and IL-6 in the experimental group were significantly lower than those of the model group, and the combination group was su- perior to the acupuncture group and Wenpi Bushen Decoction group;the differences were statistically significant （P 〈 0.05）. The levels of SOD and GSH-PX in experimental group were significantly higher than the model group,and LPO were significantly lower than that of
出处
《中国中医急症》
2017年第12期2090-2092,2123,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
河北省中医药管理局科研计划项目(2017250)
关键词
急性溃疡性结肠炎
针灸
温脾补肾方
炎症因子
氧化应激
Acute ulcerative colitis
Acupuncture
Wenpi Bushen Decoction
Inflammator~ factors
Oxidative stress
