摘要
目的观察电针足三里穴对溃疡性结肠炎(UC)大鼠骶髓后联合核(SDCN)内神经元和胶质细胞形态学反应的影响,初步探讨SDCN内神经元和胶质细胞参与针刺调控UC的中枢神经机制。方法48只雄性SD大鼠随机分为空白对照组(对照组)、溃疡性结肠炎组(UC组)、UC+电针足三里穴组(足三里穴组)和UC+电针非经穴组(非经穴组),足三里穴组根据针刺的时间又分为1、3、5d 3个亚组,每组8只大鼠。采用三硝基苯磺酸、乙醇液灌肠法诱导建立UC大鼠模型。实验结束后处死各组动物,取骶髓切片,采用抗Fos/抗GFAP双标记(分别标记神经元和星形胶质细胞)和抗OX42(标记小胶质细胞)的ABC法进行免疫组织化学染色。结果除对照组外,Fos阳性神经元、GFAP阳性星形胶质细胞和OX42阳性小胶质细胞的表达均集中对称分布于SDCN内。以UC组和非经穴组的神经元和胶质细胞的阳性免疫反应表达最高,而足三里穴组各时间点的阳性表达均较前两组明显减少(P<0.01)。电针足三里穴组各时间点比较,5d组较1d组阳性表达明显减少(P<0.05)。结论骶髓后联合核内免疫阳性神经元和胶质细胞参与了电针足三里穴对UC大鼠的抗炎作用过程;电针调节神经元和胶质细胞的功能活动与针刺时间有关。
Objective To observe the influences of electroacupuncture （EA） on the morphological changes in neurons and glia cells in sacral dorsal commissural nucleus （SDCN） of rats with ulcerative colitis （UC）, and study the mechanism of acupuncture in modulating central nervous system in UC. Methods Forty-eight male SD rats were randomly assigned to four groups., control group, UC model group, EA Tsusanli group （UC plus EA at Tsusanli acupoint） and EA non-acupoint group （UC plus EA non-acupoint group）. According to the acupuncture duration, EA Tsusanli group was divided into three subgroups;1-day, 3-day and 5-day subgroup （8 rats for each subgroup）. UC model of rats was reproduced by instillation of a solution containing ethanol and 2,4,6 ,-trinitrobenzenesulfonic acid （TNBS） into the distal colon. When the treatment was ended, all the rats were sacrificed and SDCN slides were prepared. The expressions of Fos, glial fibrillary acidic protein （GFAP） and OX42 immunoreactivity in SDCN were assessed and calculated by using single or double immuno histochemical methods. Results In the three experimental groups, the Fos-positive neurons, GFAP-positive astrocytes and OX42-positive microglia were mainly expressed in SDCN. In UC model group and EA non acupoint group, the expressions of Fos, GFAP and OX42 immunoreactivity were stronger than those in EA Tsusanli group （P〈0.01）. The expressions of Fos, GFAP and OX42 immunoreactivity in 5-day subgroup decreased obviously compared with those in 1-day subgroup （P〈0.01; P〈0.05）. Conclusions The Fos-positive neurons, GFAP positive astrocytes and OX42 positive microglia in SDCN may play an important role in the acupuncture therapy for UC, and this function may be related to the acupuncture time.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2008年第12期1454-1456,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助项目(39970888)
陕西省中医药管理局中医药科研课题基金资助项目(36)
关键词
电针
穴
足三里
结肠炎
溃疡性
骶髓后联合核
免疫组织化学
electmacupuncture
POINT ST36（ZUSANLI）
colitis, ulcerative
sacral dorsal commissural nucleus
immunohisto-chemistry
