摘要
目的观察健脾补肾利湿方对高尿酸肾病模型大鼠的抗炎、抗氧化作用。方法 SD大鼠除空白组外,其余采用酵母干粉、腺嘌呤制作高尿酸肾病模型。造模成功后随机分为模型组、健脾补肾利湿组(简称脾肾组)、别嘌醇组。治疗8周,检测大鼠血尿酸(BUA)、肌酐(SCr)、尿素氮(BUN)浓度,采用ELISA法检测模型大鼠血清IL-6、TNF-α水平,采用黄嘌呤氧化酶法、二硫对二硝基苯甲酸(DTNB)直接显色法和可见光分光光度法检测SOD、GSH-PX、CAT酶活性,采用TBA法检测MDA含量。结果与空白组比较,模型组各指标差异均有统计学意义(P<0.05,P<0.01)。与模型组比较,脾肾组BUA、SCr、BUN、IL-6、TNF-α水平降低,GSH-PX、SOD、CAT酶活性升高,MDA含量下降,差异均有统计学意义(P<0.05)。与别嘌醇组比较,脾肾组降低大鼠BUA、SCr、BUN、IL-6、TNF-α水平,升高GSH-PX酶活性,降低MDA含量,差异有统计学意义(P<0.05);在升高SOD、CAT酶活性方面,两组差异无统计学意义(P>0.05)。结论健脾补肾利湿方具有较好的抗炎、抗氧化作用,可积极影响高尿酸肾病大鼠模型的微炎症及氧化应激状态。
Objective To observe the anti-inflammation and anti-oxidation effects of＂Jianpi Bushen Lishi Recipe ＂in hyperuricemia nephropathy rats. Methods The model of hyperuricemia nephropathy was made by yeast dry powder and adenine in SD rats,and the normal rats were taken as the blank group. After successful modeling,the rats were randomly divided into the model group,Jianpi Bushen Lishi Recipe group and allopurinol group. Each group was treated with the corresponding drugs for 8 weeks. The levels of blood uric acid（ BUA）,serum creatinine（ SCr） and blood urea nitrogen（ BUN） were detected. The serum levels of IL-6 and TNF-α were detected by ELISA. The activities of superoxide dismutase（ SOD）,glutathione peroxidase（ GSH-PX） and catalase（ CAT） were detected by xanthine oxidase method,DTNB direct coloration and visible spectrophotometry,respectively. The content of malondialdehyde（ MDA） was detected by thiobarbituric acid（ TBA） method. Results There were statistically significant differences on all indicators between the blank group and model group（ P〈0. 05,P〈0. 01）. Compared with the model group,the levels of BUA,SCr,BUN,IL-6 and TNF-α were decreased in the Jianpi Bushen Lishi Recipe group,and the activities of GSH-PX,SOD and CAT were increased and the content of MDA was decreased,with statistically significant differences（ P〈0. 05）. Compared with the allopurinol group,the levels of BUA,SCr,BUN,IL-6 and TNF-α were decreased in the Jianpi Bushen Lishi Recipe group,and the activity of GSH-PX was increased and the content of MDA was decreased,with statistically significant differences（ P〈0. 05）. There were no statistically significant differences on the activities of SOD and CAT between the allopurinol group and Jianpi Bushen Lishi Recipe（ P〉0. 05）. Conclusion Jianpi Bushen Lishi Recipe shows good anti-inflammation and anti-oxidation effects,which can positively affect the micro-inflammation and oxidative stress in hyperuricemia nephropathy rats.
出处
《上海中医药杂志》
2017年第12期71-74,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市卫计委科研课题青年项目(20174Y0047)
关键词
高尿酸肾病
健脾补肾利湿方
微炎症
氧化应激
hyperurieemia nephropathy
Jianpi Bushen Lishi Recipe
micro-inflammation
oxidative stress
