摘要
目的建立二妙丸中盐酸小檗碱、盐酸黄柏碱、盐酸巴马汀、木兰花碱及苍术素等5种成分的含量测定方法。方法采用超高效液相色谱法,色谱柱:Waters BEH(2.1 mm×100 mm,1.7μm),乙腈-0.1%磷酸(每100 m L加十二烷基硫酸钠0.1 g)为流动相,梯度洗脱,流速为0.3 m L·min^(-1),盐酸黄柏碱、木兰花碱、盐酸小檗碱、巴马汀检测波长为280 nm;苍术素检测波长为340 nm;柱温:30℃。结果盐酸小檗碱、黄柏碱、巴马汀、木兰花碱及苍术素5种成分在一定范围内浓度与峰面积呈良好的线性关系;精密度,稳定性,重现性等均符合方法学要求;平均回收率在96.8%~99.3%之间,RSD均小于2.8%。结论建立的方法简便快捷,准确度高,重现性好,可为二妙丸的质量控制提供依据。
OBJECTIVE To develop an ultra-high performance liquid chromatography （UHPLC） method for simultaneous deter- mination of berberine hydrochloride, phellodendrine hydrochloride, palmatine hydrochloride, magnoflorine, and rhizoma atractylodis in Ermiao pills. METHODS The UHPLC analyses were performed on a Waters BEH C18 column （2. 1 mm × 100 mm, 1. 7 μm） eluted with acetonitrile and 0. 1% phosphoric acid（ each 100 mL containing 0. 1 g sodium dodecyl sulfate）. The flow rate was 0. 3 mL · min- 1 , the detection wavelength was set at 280 and 340 nm, and the column temperature was set at 30 ℃. RESULTS The calibration curves of the five compositions had good linear relationship in the ranges of the tested concentrations. The precision, stability and repeatability complied with the requirements of methodology. The recoveries were between 96. 8% - 99.3% respectively. The RSDs were below 2.8%. CONCLUSION The developed method is simple, sensitive, rapid and accurate. This work provides helpful information for the comprehensive quality evaluation of Ermiao pills.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2017年第23期2122-2125,共4页
Chinese Pharmaceutical Journal
