摘要
目的建立木香顺气丸中辛弗林的含量测定方法。方法采用TSK Amide80色谱柱（4.6 mm×150m,5μm）,以乙酸氨溶液13mM（pH6.7）-乙腈（v/v 17∶83）为流动相,流速为1mL·min-1,检测波长275nm。结果辛弗林的线性范围为1.6-40μg·mL^-1（r=0.999 2）;平均加样回收率为98.5%（n=9）,RSD为0.59%。结论本方法简单、迅速,辛弗林的保留因子κ满足要求,可用于木香顺气丸中陈皮药材的质量控制。
Objective To establish the quantitative method for synephrine Methods The separation was undertaken on TSKgel Amide-80（4.6mm×150m,5μm）column,the mobile phase was composed with13 mM Amonium Acetate buffer（pH6.5）and acetonitrile（v/v17∶83）,flow rate was 1 mL·mL^-1,the detection wavelength was 275 nm,the linearity of synephrine was between 1.6μg·mL^-1and 40μg·mL^-1（r=0.999 2）,and average recovery was 98.5%（n=9）RSD=0.59% Results the method was simple and fast,and the retention factorκreached the requirements Conclusion the method could be applied to quality control of Muxiangshunqi Pills
出处
《中国药事》
CAS
2014年第12期1361-1364,共4页
Chinese Pharmaceutical Affairs
