摘要
为探究苷苔Ecklonia cava岩藻聚糖硫酸酯粗品(EC-FUC)的组成结构,以产自韩国济州岛海岸的苷苔Ecklonia cava中提取的EC-FUC为原料,利用DEAE-Sepharose fast flow阴离子交换层析和Sephacryl S-300、S-400凝胶柱层析对EC-FUC进行分离纯化及分子量的测定,采用苯酚-硫酸法和明胶-Ba Cl2法分别测定纯化各组分的总糖含量及硫酸根含量,采用高效液相色谱法、红外光谱法、核磁共振波谱法分别测定各个纯化组分的单糖组成并分析多糖结构。结果表明:EC-FUC纯化后得到ECF-1、ECF-2和ECF-3组分,其总糖含量分别为41.7%、57.79%、75.78%,硫酸根含量分别为3.68%、3.41%、18.03%,回收率分别为6.27%、8.77%、16.19%,其相对分子质量分别为16 430、16 430、222 460;ECF-1单糖组成主要为葡萄糖,ECF-2单糖组成主要为甘露糖醛酸、葡萄糖醛酸和岩藻糖,ECF-3单糖组成主要为岩藻糖和葡萄糖醛酸;红外光谱分析显示,ECF-1、ECF-2和ECF-3硫酸基主要连接在岩藻糖的C2、C3位置;13C核磁共振分析显示,ECF-2中存在甘露糖醛酸,ECF-3在17.97×10-6处出现α-L-吡喃岩藻糖C6信号峰。本研究结果可为苷苔藻聚糖硫酸酯组成结构提供丰富的信息。
Crude polysaccharide was separated from fucoidan(EC-FUC) extracted from brwn seaweed Ecklonia cava sampled from coast of Cheju Island in R. O. Korea by DEAE-Sepharose fast flow anion exchange chromatography,and future purified by Sephacryl S-300,S-400 gel column chromatography. The molecular weight and content of polysaccharide and sulfate of the EC-FUC were determined by phenol sulfuric acid method and Ba Cl2 gelatin method,respectively. High performance liquid chromatography,infrared spectroscopy and nuclear magnetic resonance spectroscopy were used to analyze the structural characterization of purified components. The results showed that the purified EC-FUC was comprised of three peaks including ECF-1 with total polysaccharide of 41.7% and sulfate content of 3.68%,ECF-2 with total polysaccharide of 57.79% and sulfate content of 3.41%,and ECF-3 with total polysaccharide of 75.78%,and sulfate content of 18.03%. The yields were 6.27% in ECF-1,8.77% in ECF-2 and 16.19% in ECF-3,with molecular weight of 16 430 in ECF-1,16 430 in ECF-2 and 222 460 in ECF-3. The primary monosaccharide was shown to be glucose in ECF-1,mannuronic acid,glucuronic acid and fucose in ECF-2,and glucuronic acid and fucose in ECF-3. Infrared spectrum analysis revealed that the sulfated group was combined to C2 or C3 in the three fractions and13 C nuclear magnetic resonance(NMR) analysis indicated that there was mannuronic acid in ECF-2 and that 17.97×10-6 regions of ECF-3 was assigned to C6 α-L-fucopyranose.
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2017年第6期740-746,共7页
Journal of Dalian Ocean University
基金
国家自然科学基金资助项目(31471610)
国家海洋公益性行业科研专项(201405040)
关键词
苷苔
岩藻聚糖硫酸酯
分离纯化
结构分析
Ecklonia cava
fucoidan
isolation and purification
structural analysis
