摘要
采用高效液相色谱法,以木犀草素(3)为内标物,建立了一测多评法同时测定凤尾草中的忍冬苷(1)、野漆树苷(2)、3和芹菜素(4)。采用Zorbax Eclipse Plus C_(18)柱,以乙腈∶0.1%磷酸为流动相,线性梯度洗脱,检测波长350 nm。1、2、4与3的相对校正因子(RCF)分别为1.365、2.128、1.890,相对保留时间(RRT)分别为0.384、0.623和1.097。1~4分别在48~480、12~120、22~220和23~230 ng范围内线性关系良好,回收率分别为99.34%、98.08%、97.78%和102.12%,RSD分别1.26%、1.35%、0.79%和1.03%。利用本法与外标法分别测定10批凤尾草药材中的1~4,两种方法测定结果无显著性差异。
A quantitative analysis of multi-components by single marker （QAMS） method was established for the determination of 4 components, lonicerin （1）, rhoifolin （2）, luteolin （3） and apigenin （4） in Herba Pteridis multifidae, based on the application of HPLC and with compound 3 as the internal standard. A Zorbax Eclipse Plus C18 column was used, with the mobile phase of acetonitrile∶0.1% phosphoric acid for gradient elution and at the detection wavelength of 350 nm. The relative correction factors （RCFs） of 1, 2 and 4 were 1.365, 2.128 and 1.890, and their relative retention time （RRTs） were 0.384, 0.623 and 1.097. The calibration curves of 1—4 were linear in the ranges of 48—480,12—120, 22—220 and 23—230 ng, respectively. Their recoveries were 99.34%, 98.08%, 97.78% and 102.12%, with RSDs of 1.26%, 1.35%, 0.79% and 1.03%, respectively. Ten batches of Herba Pteridis multifidae were quantitatively determined by QAMS method and the external standard method （ESM）. The results showed that there were no significant differences between these two methods.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2017年第12期1791-1795,共5页
Chinese Journal of Pharmaceuticals
基金
国家自然科学基金资助项目(81483286
81302783)
安徽省高校省级自然科学研究重点项目(KJ2014A051)
安徽省自然科学基金项目(10040606Q13
1308085MC43)
安徽省教育厅重点和重大项目(KJ2016SD16)
安徽省大学生创新课题(2017S10879004)
关键词
一测多评
凤尾草
高效液相色谱
含量测定
quantitative analysis of muti-components with a single marker （QAMS）
Herba Pteridis multifidae
HPLC
content determination
