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化瘀祛痰方对高脂血症大鼠肝脏mTOR介导自噬相关基因的影响 被引量:7

Effect of Huayu Qutan Recipe on Liver Mediated Autophagy Related Gene mTOR in Hyperlipidemia Rats
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摘要 目的:利用PCR array技术探讨化瘀祛痰方药对高脂血症大鼠肝脏mTOR介导自噬相关基因的影响。方法:30只SD大鼠随机分为正常对照组、高脂血症组、化瘀祛痰方组。正常对照组给予普通大鼠饲料喂饲,高脂血症组、化瘀祛痰方组予高脂饲料喂饲,造模60 d后,化瘀祛痰组给予化瘀祛痰方煎剂10 m L/次,1次/d,灌胃30d(该剂量是根据人与大鼠的等效剂量进行换算所得)。全自动生化分析仪检测大鼠血清TC、TG、LDL-C、HDLC含量,HE及油红O染色观察肝脏形态及肝脏脂质沉积情况,PCR array技术分析化瘀祛痰方对高脂血症大鼠肝脏mTOR介导自噬相关基因的影响。结果:高脂血症组血脂(TC、LDL-C)含量升高,出现肝脏脂质沉积及脂肪空泡;化瘀祛痰方组血脂(TC、LDL-C)降低,肝脏脂滴减少。PCR array结果发现以mTOR为中心的不同作用途径基因Akt1、Bcl2、lgf1、Mapk14、Mapk8、Mtor、Becn1(brclin1)在3组比较发生差异性改变。结论:化瘀祛痰方可通过调控mTOR介导自噬相关基因使高脂血症大鼠肝脏自噬增强,改善高脂血症大鼠肝脏脂质损伤。 Objective : To explore the effect of Huayu Qutan Recipe on liver mTOR induced hyperlipidemia rats by auto- phagy related genes using PCR array technology. Methods : Thirty SD rats were randomly divided into normal control group, hyperlipidemia group and Huayu Qutan Recipe group. Normal control group was given ordinary rat feeding. The hyperlipidemia group and Huayu Qutan Recipe group were given high fat diet feeding. 60 days after modeling, Huayu Qu- tan Recipe group was given Qutan Huayu Decoction 10 mL for each time, once a day, by irrigation stomach way for 30 days (the dose was based on the equivalent dose of human and rats conversion income). Full automatic biochemical ana- lyzer was used to measure the rats' serum TC, TG, LDL - C and HDL - C contents. He staining and oil red O staining were used to observe the morphology of liver and liver lipid deposition and PCR array analysis for the effect of Huayu Qu- tan Recipe on hyperlipidemia rats' liver mTOR mediated autophagy related genes. Results : In hyperlipidemia group, ser- um lipid (TC, LDL - C) content increased and there was lipid deposition in liver and fat vacuoles. The Huayu Qutan Recipe group's blood lipid (TC, LDL - C) and reduced liver lipid drops. Array PCR results showed that mTOR, Bcl2,, lgfl, Mapkl4, Mapk8, Mtor, Becnl and Aktl (brclinl) in different action pathway genes were different in the three groups. Conclusion:Huayu Qutan Recipe can make liver autophagy in hyperlipidemia rats enhanced by mTOR medi- ated regulation of autophagy related genes and improve hyperlipidemia rats' liver lipid injury.
出处 《中华中医药学刊》 CAS 北大核心 2017年第12期3143-3146,I0033,I0034,共6页 Chinese Archives of Traditional Chinese Medicine
基金 辽宁省自然科学基金项目(2015020394) 辽宁省高等学校优秀人才支持计划项目(LR2015041) 辽宁省科学技术计划项目(2014225004)
关键词 化瘀祛痰方 高脂血症 MTOR 自噬 PCR ARRAY Huayu Qutan Recipe hyperlipemia mTOR autophagy PCR array
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