摘要
目的:观察脂质异常家兔抗氧化能力及血管细胞黏附因子-1(VCAM-1)的变化以及血脂康的干预。方法:采用高脂饲料喂养法复制血脂异常家兔模型,检测各组家兔血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平,并用酶联免疫吸附(ELISA)法测定家兔血清VCAM-1的含量。结果:高脂血症组家兔血清TC、TG、LDL-C、MDA、VCAM-1水平升高,SOD水平降低,与之相比,血脂康组家兔血清TC、TG、LDL-C、MDA水平降低(P<0.05),家兔血清中VCAM-1的含量降低(P<0.01),SOD水平明显升高(P<0.05)。结论:脂质异常可致家兔抗氧化能力降低,引起脂质过氧化,损伤内皮细胞,导致VCAM-1含量升高。血脂康可改善脂质异常家兔血清抗氧化能力、清除自由基、抗脂质过氧化损伤并下调VCAM-1的表达,提示其具有保护内皮细胞功能的作用。
Objective ： To investigate the effects of Xuezhikang on the anti - oxidation resistance and the level of vascular cellular adhesive molecule （ VCAM - 1 ） in the dyslipidemic rabbits. Methods ： Hyperlipidemic rabbit model was obtained by feeding high fat diet. Then, the dyslipidemic rabbits in treatment group were treated with Xuezhikang. Four weeks later, the blood levels of cho- lesterol, triglyceride, LDL - C, MDA and SOD were measured by RIA in rabbits of all groups. The expression of VCAM - 1 was detected by ELISA. Results ： The levels of TC, TG, LDL - C and MDA in Xuezhikang group decreased while the level of SOD increased, compared with the model group（ P 〈 0.05 ）. The result of ELISA showed that Xuezhikang could decrease the VCAM -1 strngth （P 〈 0.01 ）. Conclusion： Xuezhikang can inhibit lipid＇ s peroxidation damange and inhibit the expression of VCAM - 1 in the hyperlipidemic rabbit. The results indicate that Xuezhikang can protect the vascular endothelin cells.
出处
《天津药学》
2010年第1期1-3,共3页
Tianjin Pharmacy
