摘要
目的:探讨峨参内酯与5-FU联用对人结肠癌SW480三维立体多细胞球模型的作用及其机制。方法:采用CCK8法检测峨参内酯及峨参内酯与5-FU联用对SW480三维立体多细胞球模型的抑制作用;采用流式细胞仪观察分析峨参内酯与5-FU联用对SW480三维立体多细胞球模型细胞周期和细胞凋亡的影响;采用蛋白质印迹(Western blot)技术检测分析峨参内酯与5-FU联用对SW480三维立体多细胞球模型细胞中FAK、COX2蛋白表达的影响。结论:峨参内酯对人结肠癌SW480三维立体多细胞球模型有明显的抑制作用,呈现出一定的药物剂量依赖性,其半数致死量分别为26.82μg/m L;峨参内酯与5-FU联用时,细胞活力显著降低,且随着峨参内酯浓度的加大而逐渐降低;5-FU可使SW480细胞周期停止在S期,峨参内酯可使SW480细胞周期停止在S期、G2/M期,两种药物配合使用,可使SW480细胞周期停止在S期、GA/M期并最终诱导细胞凋亡,并且能够明显抑制细胞中FAK、COX2两种蛋白的表达。
Objective ： To investigate the effects of Anthricin combined with fluorouracil and the mechanism on SW480 three -muhicellular spheroids models,providing the basic research data for further elucidate the anti -tumor mechanism of Anthriscus sylvestris. Methods ： We established the SW480 three - dimensional multi - cellular spheroids model and tested Anthricin combined with fluorouracil in the inhibition effects on SW480 three - dimensional muhicellular spheroids models by CCK8 method. We tested Anthricin combined with fluorouracil in the influnences of the cell cycle and apopto- sis on SW480 three - dimensional multicellular spheroids models by the flow cytometry and detected the influences of An- thricin combined with fluorouracil in expressions of FAK and COX2 protein in SW480 three - dimensional muhicellular spheroids models. Results ： Anthricin could obviously inhibit SW480 three - dimensional multicellular spheroids models and it showed a certain dose - dependent. The half lethal dose was 26.82 μg/mL. Anthricin combined with fluorouracil could significantly decrease the activity of SW480 three - dimensional muhicellular spheroids models and the activity would decrease with the Anthricin concentration increase. 5 - FU could make SW480 cells＇ cycle be arrested in S phase and Anthricin could make their cell cycle be arrested in S phase and G2/M phase. Anthricin combined with fluorouracil could make SW480 cells＇ cycle be arrested in S phase and G2/M phase and ultimately induce cell apoptosis, and they could obviously inhibit the expressions of FAK and COX2 in SW480 cells.
出处
《中华中医药学刊》
CAS
北大核心
2017年第12期3134-3137,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81374022)
关键词
峨参内酯
5-FU
三维立体多细胞球
结肠癌
Anthricin
5 - FU
three - dimensional multi - cellular spheroids
colon cancer
