摘要
目的探讨葛根素对人胰腺癌细胞株PANC-1的作用及其机制。方法取对数生长期的PANC-1细胞,分别用不同浓度的葛根素(0、50、100、200μmol/L)干预48 h后,利用CCK-8检测PANC-1细胞增殖水平;Annexinv-FITC/PI双染流式细胞术检测细胞凋亡;Western blot检测Fas、Fas-L、Bax、Bcl-2、Caspase-7表达水平。结果葛根素对胰腺癌PANC-1细胞增殖的抑制呈浓度依赖性;不同浓度的葛根素干预PANC-1细胞48 h后,细胞凋亡率分别为2.47%±0.64%、6.37%±0.71%、8.33%±0.90%、12.40%±1.01%,实验组与对照组相比,差异均有统计学意义(P<0.01);Western blot实验表明,葛根素对胰腺癌PANC-1细胞干预48 h后,随着葛根素浓度的增加,细胞中Fas、Fas-L、Caspase-7、Bax蛋白的表达量上调,Bcl-2的表达量下调,实验组与对照组相比,差异均有统计学意义(P<0.05)。结论葛根素可通过激活Fas/Fas-L信号通路诱导PANC-1细胞增殖抑制和凋亡。
Objective To investigate the effect and mechanism of puerarin on human pancreatic cancer cell line PANC-1. Methods PANC-1 cells of logarithmic growth phase were treated with different concentrations of puerarin（ 0,50,100 and 200 μmol/L） for 48 h; CCK-8 was used to detect the proliferation level of PANC-1 cells. Apoptosis was detected by Annexinv-FITC/PI double staining flowcytometry. Western blot was used to detect the expression of Fas,Fas-L,Bax,Bcl-2 and Caspase-7. Results Puerarin inhibited the proliferation of pancreatic cancer PANC-1 cells in a concentration-dependent manner. When different concentrations of puerarin interfered PANC-1 cells for 48 h,the apoptosis rate of cells respectively was 2. 47% ± 0. 64%,6. 37% ± 0. 71%,8. 33% ± 0. 90%,and 12. 40% ± 1. 01%,the difference between experimental group and control group being statistically significant（ P〈0. 01）. Western blot experiment showed that,after the 48 h intervention for PANC-1 of pancreatic cancer cell line,the expression of Fas,FasL,Caspase-7 and Bax protein increased with the increase of puerarin concentration,while the expression of Bcl-2 protein decreased,the difference between experimental group and control group being statistically significant（ P〈0. 05）.Conclusion Puerarin can induce PANC-1 cell proliferation inhibition; Apoptosis by activating Fas/Fas-L signaling pathway.
出处
《实用药物与临床》
CAS
2017年第11期1244-1248,共5页
Practical Pharmacy and Clinical Remedies
