摘要
目的:过表达microRNA-137(miR-137)对体外人胰腺癌增殖能力的影响及其机制初探。方法:将胰腺癌PANC-1和As PC细胞分为miR-137 mimics转染组和对照组,并通过qRT-RCR检测转染效率;利用流式细胞仪检测细胞凋亡率和周期阻滞变化,CCK-8实验、平板克隆实验检测miR-137对胰腺癌细胞增殖能力的影响,qRT-RCR、Western blot等实验检测miR-137对PTN的mRNA及蛋白表达的影响。结果:上调miR-137表达后,PANC-1、As PC胰腺癌细胞凋亡率增加(P<0.05)、周期阻滞于G1期比例增加(P<0.05);CCK8及平板克隆实验结果均显示过表达miR-137后,胰腺癌细胞增殖能力降低PTN表达显著下调。结论:miR-137在胰腺癌细胞中的表达水平影响抑制胰腺癌的增殖能力,其机制可能与抑制PTN的表达有关。
Objective： To investigate the effect of miR-137 on the proliferation of pancreatic cancer cells and to study the mechanism. Methods： The pancreatic cancer PANC-1 and As PC cells were divided into miR-137 mimics transfection group and control group,and the transfection efficiency was detected by qRT-RCR. Flow cytometry was used to detect the apoptosis rate and the changes of cell cycle arrest,The effect of miR-137 on the proliferation of pancreatic cancer cells was detected by CCK-8 assay and plate cloning assay. The effects of miR-137 on the mRNA and protein expression levels of PTN were measured by PCR and Western blot. Results： miR-137 up-expression effectively inhibited the apoptosis rate and controled cell cycle arrest of pancreatic cancer cell（ P 〈0. 05）. The proliferation ability of PANC-1 and As PC cells decreased significantly after over-expression of miR-137（ P〈 0. 05）.Meanwhile miR-137 over-expression decreased the expression level of PTN（ P〈 0. 05）. Conclusion：miR-137 inhibits the proliferation of pancreatic cancer cells possibly through down-regulating the PTN expression level.
出处
《贵州医科大学学报》
CAS
2017年第11期1241-1246,共6页
Journal of Guizhou Medical University
基金
国家国际科技合作专项资助项目(2014DFA31420)
国家自然科学基金资助项目(81560477)
贵州省科学技术厅-贵州医科大学附属医院联合基金[黔科合LH字(2016)7229号]
贵州省第四批人才基地基金资助[黔省专合字(2012)94号]
贵州省科学技术厅-贵阳医学院院士工作站肝胆外科分站[黔科合院士站(2015)4013]
贵州医科大学肝胆胰脾重点实验室项目资助
