摘要
目的:探讨硫酸耐而蓝(NBS)探针共振瑞利散射法(RRS)测定糖精钠(SC)含量。方法:先在弱酸性介质中加入SC,与硫酸耐而蓝形成离子缔合物,采用NBS探针RRS法测定SC含量,记录产生新的RRS光谱,RRS光谱的最大散射峰,RRS强度变化有良好的线性关系的波长、SC浓度范围、该法检出限及回收率范围。结果:最大散射峰位于802 nm,另在507、400 nm有两个较弱的散射峰;802 nm处SC浓度在0.5~25 mg/L范围内RRS强度变化有良好的线性关系,该法检出限为0.17 mg/L,回收率范围为90.2%~102.8%。结论:硫酸耐而蓝-SC体系的RRS方法可用于果汁中SC的测定。
Objective： To investigate resonance Rayleigh scattering（ RRS） method for the determination of Saccharin with Nile blue sulphate. Methods： Adding Saccharin in weak acid medium,reacted with Nile blue sulphate to form a ion-associated complex and determining the content of SC; which led to a distinctly enhanced RRS and recording new RRS spectrum and the scattering; RRS strength variation showed fine linear relationship wavelength,SC concentration scale,and limit of detection of this method and recovery rate scale. Results： There is a new strong RRS peak located at 802 nm and there were two smaller scattering peaks at 507 nm and 400 nm. The intensities of RRS are proportional to the concentration of Saccharin in the range of 0. 5 ~ 25 mg/L. The detecttion limit is 0. 17 mg/L.The recovery rate scale was 90. 2% to 102. 8%. Conclusion： The method can be applied to the determination of saccharin in juice.
出处
《贵州医科大学学报》
CAS
2017年第11期1283-1286,共4页
Journal of Guizhou Medical University
基金
贵州医科大学大学生创新创业项目(201610660002)
贵阳市科技计划项目[筑科合同(2016001)001号]
关键词
糖精钠
硫酸耐而蓝
共振瑞利散射
离子缔合微粒
光谱分析
Saccharin
nile blue sulphate
resonance rayleigh scattering
ion-associated complex
spectrum analysis
