摘要
目的:探讨Th17及其相关因子在慢性牙周炎发病中的作用。方法:选择46例牙周科患者分为4组:慢性牙周炎活动型位点组、慢性牙周炎静止型位点组、牙龈炎组和健康对照组,应用酶联免疫法检测各组龈沟液中IL-17、RORC2、IFN-γ和IL-4的含量并进行分析。结果:IL-17和RORC2的表达趋势相似,活动型位点组明显高于其他3组,静止型位点组明显高于健康对照组;健康对照组的IFN-γ明显低于其他3组;活动型位点组IL-4明显低于其他3组;IL-17和RORC2呈正相关,IL-17和IL-4呈负相关;IL-17、RORC2、IFN-γ与临床指标BI、PD和AL呈正相关,IL-4与BI、PD和AL呈负相关。结论:Th17在慢性牙周炎活动型中占优势,随着附着丧失增加,龈沟液中含量升高;IFN-γ可能参与了牙龈炎症和非炎症的状态转化。
Objective：To study the correlation of Th17 and related factors in the gingival crevicular fluid（GCF）of the patients with different periodontal inflammatory status.Methods：Forty-six patients were divided into four groups：periodontitis active site group,non-active site group,gingivitis group,and healthy group.IL-17,RORC2,IFN-γ,and IL-4 in GCF were detected by enzyme-linked immunosorbent assay（ELISA）.Results：The expression trend of IL-17 and RORC2 were similar.The levels of IL-17 and RORC2 in active site group were significantly higher than those of other three groups,and the levels of non-active site group were significantly higher than that of healthy group（P0.05）.The expression of IFN-γin healthy group was significantly lower than those of other three groups（P0.05）.In active periodontal site group,the expression level of IL-4 was significantly lower than those of other three groups（P0.05）.IL-17 level was positively correlated with RORC2,and negatively correlated with IL-4（P0.05）.The levels of IL-17,RORC2 and IFN-γwere positively correlated with those of BI,PD and AL.The level of IL-4 was negatively correlated with those of BI,PD and AL（P0.05）.Conclusion：The expression of Th17 was strong in active site group,and it increased with the aggravation of attachment loss.IFN-γmight be involved in the transformation of gingival inflammation and non-inflammation status.
出处
《口腔医学研究》
CAS
北大核心
2017年第11期1161-1164,共4页
Journal of Oral Science Research
基金
湖北省卫生计生科研基金(编号:WJ2015MB040)
