摘要
目的:探讨不同摄取量的尼古丁对大鼠正畸过程牙周改建的影响。方法:选择120只雄性Wistar大鼠并将其随机分为四组:A组-空白对照,B组-正畸模型,C组-正畸并0.01 mg/m L尼古丁给药,D组-正畸并1 mg/m L尼古丁给药。分别于实验开始后第1、3、7、14、21天通过Micro-CT和HE染色观察模型牙齿移动距离和牙周组织改变并通过ELISA实验检测IL-17的表达。结果:Micro-CT扫描显示:正畸建模组相对于空白对照组在牙移动距离、骨体积分数、骨密度等指标均有明显变化,变化最大幅度发生在D组,B、C两组之间的差异没有统计学意义(P>0.05)。21天,D组移动距离达到0.80±0.06 mm,明显高于B、C组(P<0.05)。相较于空白对照组(A组),B、C、D三组Micro-CT测量的骨体积分数、骨密度、骨小梁厚度均降低,D组骨密度值降至1108.36±8.86mg/cm3。HE染色结果显示:D组在21天时破骨细胞增多并出现牙根吸收陷窝伴牙周膜纤维排列混乱;ELISA检测显示B、C组IL-17的含量在第7天时达到峰值,D组则在14天含量最高。结论:高浓度的尼古丁可加速正畸牙齿的移动速度及牙槽骨吸收,增加牙周组织中的破骨细胞及IL-17表达水平。
Objective： The study the effect of differentdosis of nicotine on the periodontium during orthodontic treatment in adult rats. Methods： 120 cases of Wistar male rats were segmented into four groups stochastically： A-control; B-orthodontic model group;C-orthodontic ＋ 0.01 mg/kg nicotine group; D-orthodontic＋1 mg/kg nicotine group. Micro-CT was used to measure tooth movement and the changes on microstructure of trabecular, the variety of periodontal microstructures and expression of IL-17 on day 1, 3, 7, 14 and 21 were detected and compared. Results： Micro-CT scanning showed that the orthodontic groups changed largely on observed indicators,such as tooth movement, BVF, and BMD, when compared to control groups. There were no significant changes between B and C groups（p〈0.05）, D group changed mostly. The tooth movement of D group reached 0.80 ± 0.06 mm, which was obviously higher than B and C groups at day 21（p〈0.05）. The BVF, BMD and Tb.Th in all groups lessened apart from A group. HE staining of A-D groups indicated that the ligament of D group was disordering and more osteoclasts.The expression level of IL-17 reached its peak at day 7 in B, C groups,but it was at day 14 for D group. Conclusion： High-dose nicotine can not only accelerate the speed of tooth movement and the absorption of alveolar bone;in addition, but also increase the quantity of osteoclast and IL-17 expression.
作者
孙鲁旻
杨东红
王凯
房玉镇
韩兆国
叶之慧
SUN Lu-min;YANG Dong-hong;WANG Kai;FANG Yu-zhen;HAN Zhao-guo;YE Zhi-hui(Department of Orthodontic,Stomatological Hospital Affiliated to Jiamusi University,Jiamusi,Heilongjiang,154004,China;Molecular Imaging Research Center,The fourth affiliated hospital of Harbin medical university,Harbin,Heilongjiang,150000,China;Department of Orthodontic,Ji＇nan Third People＇s Hospital,Ji＇nan,Shandong,250000,China)
出处
《现代生物医学进展》
CAS
2018年第13期2448-2451,2441,共5页
Progress in Modern Biomedicine
基金
黑龙江省教育厅基础研究项目(2016-KYYWF-0611)
