摘要
目的评价慢性萎缩性胃炎N-甲基-N'-硝基-N-亚硝基胍(MNNG)复合造模法的稳定性及对动物肝脏的影响。方法 60只SPF级健康雄性SD大鼠随机分为正常组8只及造模1组、造模2组各26只。造模1组用MNNG复合高盐热淀粉糊法、造模2组采用MNNG复合乙醇灌胃法制备慢性萎缩性胃炎大鼠模型,正常组正常饲养。造模时间28周。造模后比较造模1组、造模2组大鼠胃黏膜病理程度,血清胃蛋白酶原Ⅰ(PGⅠ)、胃蛋白酶原Ⅱ(PGⅡ)及肝脏病理改变。结果造模1组大鼠24周出现腺体异型增生,26周出现灶性肠化生。造模2组大鼠26周出现个别腺体异型增生,28周出现灶性肠化生。模型1组死亡率为26.9%,模型2组为15.4%。造模后造模1组、造模2组大鼠胃黏膜病理改变差异无统计学意义(P>0.05)。与正常组比较,造模1组、造模2组血清PGⅠ、PGⅠ/PGⅡ降低(P<0.05)。28周造模1组及造模2组大鼠肝脏细胞均出现水样变性。结论慢性萎缩性胃炎MNNG复合造模法造模时间较长,模型死亡率较高,并且对大鼠肝脏组织有一定影响。
Objective To evaluate the stability of a compound modeling method of chronic atrophic gastritis with N-methyl-N＇-nitro-N-nitrosoguanidine（ MNNG） and its effect on animal hepatocytes. Methods Sixty specificpathogen-free healthy male Sprague Dawley rats were randomly divided into a normal group（ n = 8）,a model group 1（ n = 26） and a model 2 group（ n = 26）. The model group 1 adopted MNNG combined with high salt hot starch paste method and the model 2 group adopted MNNG combined with ethanol gavage method to prepare rat model with chronic atrophic gastritis. The normal group received normal feeding. Rats underwent modeling for twenty eight weeks. Gastric mucosa pathological degree,serum levels of pepsinogen Ⅰ（ PG Ⅰ） and pepsinogen Ⅱ（ PG Ⅱ）,and the change of liver pathology of rats in the model group 1 and the model 2 group were compared after modeling. Results Rats in the model group 1 had gland hyperplasia at the 24 week,and had focal intestinal metaplasia at the 26 week.Rats in the model 2 group had individual gland hyperplasia at the 26 week,and had focal intestinal metaplasia at the28 week.,The mortality rate was 26. 9% in the model 1 group,and 15. 4% in the model 2 group. After modeling,there was no statistically significant difference in gastric mucosa pathological changes between rats in the model 1 and in the model 2 group（ P 0. 05）. Compared with those in the normal group,serum levels of PGⅠ,and PGⅠ/PGⅡ in the model 1 group and model group 2 decreased（ P 0. 05）. At the 28 week of modeling,hepatocytes of both rats in the model 1 group and model group 2 appeared hydropic degeneration. Conclusion The MNNG compound modeling method of establishing model of chronic atrophic gastritis needs longer modeling time. It is with high mortality,and has a certain effect on the liver tissue of rats.
作者
任金刚
杨洋
瞿先侯
尹璐
杨敏
严宁娟
刘涛
魏玮
REN Jingang;YANG Yang;QU Xianhou;YIN Lu;YANG Min;YAN Ningjuan;LIU Tao;WEI Wei(Beijing Chinese Medicine Hospital, Capital Medical University, Beijing 100010;Wangjing Hospital, China Academy of Chinese Medical Sciences;Beijing University of Chinese Medicine)
出处
《中医杂志》
CSCD
北大核心
2017年第22期1961-1964,共4页
Journal of Traditional Chinese Medicine
基金
2015年中医药行业科研专项(201507001-09)
北京市科技计划(Z161100001816016)
功能性胃肠病中医诊治北京市重点实验室
