摘要
目的 评价复合造模法构建慢性萎缩性胃炎(chronic atrophic gastritis, CAG)及胃癌前病变(precancerous lesions of gastric cancer, PLGC)大鼠模型的可行性。方法 将60只SPF级雄性SD大鼠随机分为对照组(n=10)及模型组(n=50)。对照组正常饲养,模型组用N-甲基-N′-硝基-N-亚硝基胍(MNNG)自由饮用+水杨酸钠隔日灌胃+脱氧胆酸钠隔日灌胃+不定期禁食复合造模法构建慢性萎缩性胃炎大鼠模型,造模时间为6个月。于造模第3,4,5,6个月比较模型组与对照组大鼠胃黏膜病理程度,造模后采血,采用ELISA检测血清胃蛋白酶原Ⅰ(PGⅠ)、胃蛋白酶原Ⅱ(PGⅡ)及胃泌素17(G-17)。结果 模型组大鼠复合刺激4个月开始出现胃腺体萎缩,5个月出现纤维化,6个月出现胃腺消失及异型增生,1只出现癌变。模型组6只大鼠异常死亡,造模死亡率为12%。与对照组比较,模型组血清PGⅠ升高(P<0.05),PGⅠ与PGⅡ比值(PG R)降低(P<0.05),G-17降低(P<0.05)。结论 采用复合造模法制备大鼠CAG模型成功率高,结果较稳定,但制备时间较长,且由于大鼠个体差异,造模进程难以完全统一,所有大鼠均出现胃黏膜萎缩,部分大鼠出现PLGC特征,个别大鼠出现癌变。
Objective To evaluate the composite modeling method for constructing a rat model of chronic atrophic gastritis(CAG)or precancerous lesions of gastric cancer(PLGC).Methods Sixty SPF grade male SD rats were randomly divided into a control group(n=10)and a model group(n=50).The rats in control group were fed normally,while the CAG rats in model group were established with a composite modeling method using N-methyl-N′-nitro-N-nitrosoguanidine(MNNG)for free drinking,sodium salicylate and sodium deoxycholate for intragastric administration every other day,and irregular fasting.The treatment lasted 6 months.At the 3rd,4th,5th,and 6th month of modeling,the pathological degree of the gastric mucosa was compared between control group and model group.After modeling,rat blood was collected to detect the serum levels of pepsinogenⅠ(PGⅠ),pepsinogenⅡ(PGⅡ),and gastrin 17(G-17)by ELISA.Results The rats in model group showed atrophy of gastric glands at month 4,fibrosis at month 5,disappearance of gastric glands and atypical hyperplasia at month 6,and one rat developed cancer,with a mortality rate of 12%.Compared with control group,serum PGⅠwas increased(P<0.05),PGⅠ/PGⅡ(PG R)was decreased(P<0.05),and G-17 was decreased in in model group(P<0.05).Conclusion The composite modeling method shows a high successful rate to prepare the rat CAG model,and the results are stable,but the preparation time is relatively long.Due to the individual difference,the modeling process of rats is difficult to completely unify.All rats develop gastric mucosal atrophy after composite modeling treatment,some rats develop PLGC,and a few rats develop gastric cancer.
作者
刘雨溪
王璐
龙凯花
刘洋
靳景瑞
张红
LIU Yuxi;WANG Lu;LONG Kaihua;LIU Yang;JIN Jingrui;ZHANG Hong(Institute of Chinese Medicine,Shaanxi Academy of Traditional Chinese Medicine,Xi′an 710003,China;College of Pharmacy,Shaanxi University of Traditional Chinese Medicine;Second Clinical Department,China Medical University)
出处
《山西医科大学学报》
CAS
2024年第2期170-174,共5页
Journal of Shanxi Medical University
基金
国家中医药管理局科技司科研项目
陕西省重点研发计划项目(2023-YBSF-525)
陕西省中医药管理局科研项目(SZY-KJCYC-2023-045)
陕西省中医药管理局重点实验室建设项目(“秦药”研发重点实验室)。
关键词
慢性萎缩性胃炎
癌前病变
复合造模法
水杨酸钠
脱氧胆酸钠
N-甲基-N′-硝基-N-亚硝基胍
chronic atrophic gastritis
precancerous lesions of gastric cancer
composite molding method
sodium salicylate
sodium deoxycholate
N-methyl-N′-nitro-N-nitrosoguanidine
