摘要
目的探讨血清Dickkopf同源物1(DKK1)、甲胎蛋白(AFP)单独或联合检测原发性肝癌的临床意义。方法收集27例原发性肝癌患者(原发性肝癌组)、43例肝硬化患者(肝硬化组)的血清进行检测,采用酶联免疫吸附试验检测血清DKK1浓度,采用电化学发光免疫法检测血清AFP浓度,应用Logistic回归和ROC曲线进行分析,建立原发性肝癌诊断的预测模型。结果原发性肝癌组血清AFP、DKK1水平明显高于肝硬化组;AFP和DKK1单独检测的灵敏度分别为55.6%和77.8%,特异度均为88.4%;二者联合检测的灵敏度为81.5%,特异度为93.0%,特异度明显高于单项指标检测结果,差异有统计学意义(P<0.05)。AFP和DKK1的ROC曲线下面积(AUC)分别为0.849和0.893,二者联合检测的AUC为0.935。结论血清DKK1可作为诊断原发性肝癌的血清标志物,联合AFP可提高诊断效能,在原发性肝癌诊断中具有重要的临床价值。
Objective To investigate the clinical significance of single and combined detection of Dickkopf-1（DKK1） and alpha fetoprotein（AFP） in the diagnosis of primary hepatic carcinoma（PHC）.Methods Serum samples were collected from 27 patients with primary hepatic carcinoma and 43 patients with hepatocirrhosis.The serum level of DKK1 was detected by enzyme-linked immunosorbent assay（ELISA）,while the serum level of AFP was detected by electroch emiluminescence immunoassay（ECLIA）.Logistic regression and ROC curve were utilized to analyze,furthermore,to set up prediction models for the diagnosis of primary hepatic carcinoma.Results The serum level of AFP and DKKI in patients with PHC were higher than those with hepatocirrhosis.The sensitivity of AFP and DKK1 was 55.6% and 77.8% respectively,the specificity of both was 88.4%.In contrast,the sensitivity of combined detection was 81.5%,specificity was up to 93.0%,significantly higher than single detection.The area under the ROC curve（AUC） of AFP and DKK1 was 0.849 and 0.893 in turn,and the AUC of combined detection was 0.935.Conclusion DKK1 can be used as serum marker for the diagnosis of PHC,and the combined determination of DKK1 and AFP can improve diagnostic performance,which shows important clinic value in diagnosis of primary hepatic carcinoma.
出处
《中国现代医药杂志》
2017年第10期1-3,共3页
Modern Medicine Journal of China
基金
2014年株洲市科技计划项目
关键词
原发性肝癌
甲胎蛋白
DKK1
Primary hepatic carcinoma Alpha-fetoprotein Dickkopf-1
