摘要
探讨表面活性剂TritonX-100对桦褐孔菌液体深层发酵产活性多糖的影响,利用醇沉法得到胞外多糖和胞内多糖,通过测定菌丝体量和胞内外多糖产量,明确TritonX-100对多糖的促进作用。进一步探讨TritonX-100的最佳添加时间及浓度,得出在第0d添加0.1%TritonX-100,促进效果最显著。多糖的DPPH自由基清除率测定结果表明,TritonX-100也促进桦褐孔菌多糖的抗氧化活性。通过对多糖化学成分以及单糖组成的分析,证明多糖中的糖、蛋白含量及单糖的组成与DPPH自由基清除率具有一定关联性。以上结果为进一步深入研究桦褐孔菌多糖奠定理论基础,从而建立一种桦褐孔菌发酵多糖的高产、高活性的液体深层发酵制备技术。
This study aims to discuss the effect of surfactant TritonX-100 on the production of active polysaccharides produced from Inonotusobliquus under submerged liquid fermentation.Extracellular polysaccharide（EPS）and intracellular polysaccharide（IPS1,IPS2）were obtained with the alcohol precipitation method,and the promoting effect of TritonX-100 on polysaccharide was determined by measuring the mycelium quantity and yield of extracellular and intracellular polysaccharides.The best time and concentration for adding TritonX-100 were further studied.It is found that the promoting effect is most significant by adding 0.1% TritonX-100 on Day 0.The measurement result of DPPH free radical clearance ratio of polysaccharide indicates that TritonX-100 also can promote the antioxidant activity of I.obliquus polysaccharide.The analysis of polysaccharide chemical components and monosaccharide composition indicates that the saccharide, protein content and monosaccharide composition of polysaccharide have certain relevance to DPPH free radical clearance ratio.The conclusions presented above lay a theoretical foundation for further study on inonotus obliquus polysaccharide,based on which a submerged liquid fermentation technology of inonotus obliquus polysaccharide of high yield and high activity can be developed.
出处
《浙江理工大学学报（自然科学版）》
2017年第6期888-892,共5页
Journal of Zhejiang Sci-Tech University(Natural Sciences)
基金
浙江省自然科学基金项目(LY16B020013)
