摘要
试验研究了碳源、氮源、琼脂、天然物质在桦褐孔菌菌丝体液体培养基中的配比。结果表明:培养基配方以葡萄糖20.0 g/L、淀粉50.0 g/L、蛋白胨3.5 g/L、KH2PO43.0 g/L、MgSO41.5 g/L为最佳,菌丝生物量达0.181 2 g/100 mL。在最佳培养基配方基础上,桦褐孔菌菌丝体摇床培养5 d、培养液装量为容器的1/3时菌丝体生物量达到最大值,为0.041 4 g/50 mL。
The effects of different carbon sources, nitrogen sources, agar, natural substance, culture time and volume of culture solution on liquid culture of lnonotus obliquus were studied. The results indicated that the most suitable culture medium was glucose 20.0 g/L, starch 50.0 g/L,peptone 3.5 g/L, KH2PO4 3.0 g/L and MgS04 1.5 g/L.The biomass of dry mycelium was 0.181 2 g/100mL. When the culture medium was optimum, culture time was 5 days,and the volmne of culture solution was 1/3, the biomass of mycelium reached the highest, 0.041 4 g/50mL
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2006年第4期358-360,368,共4页
Journal of Jilin Agricultural University
基金
吉林省教育厅资助项目[吉教科合字(2004)第5号]
延边大学资助项目[延大科合字(2004)第12号]
关键词
桦褐孔菌
菌丝
液体培养
Inonotus bliquus
mycilium
liquid culture
