摘要
为粒用高粱资源的科学利用和新品种选育提供参考,利用SSR分子标记对156份全国籽粒高粱进行全基因组基因分型。结果表明：33个SSR分子标记的扫描共获得186条多态性DNA条带。多态指数（PIC,polymorphism information content）值为0.01~0.73,平均值为0.45。材料间遗传相似系数GS（Genetic Similarity）变异范围为0.15~0.9,平均值为0.53。NJ（Neighbor Joining）聚类分析和主坐标分析（PCOA,Principal-Coordinates Analysis）将156份高粱资源划分为3个类群：类群I为来源于贵州、四川和云南的56份南方高粱;类群Ⅱ包含的48份全部是北方高粱,来源于辽宁、内蒙古、山西、北京和河北等地;类群Ⅲ包含52个南方和北方的高粱,来源于黑龙江、吉林、陕西、山东、湖北和云南等地。
The genome of 156 grain sorghum germplasm resources was determined by SSR molecular marker to provide a reference for scientific utilization of grain sorghum germplasm resources and breeding of new grain sorghum varieties.Results：186polymorphism DNA bands are obtained by 33 SSR molecular markers.The polymorphism information content is 0.01~0.73,with an average of 0.45.The variation range of genetic similarity is 0.15~0.9with an average of 0.53 among different germplasm resources.156 grain sorghum germplasm resources are divided into three groups by neighbor-joining（NJ）and principalcoordinates analysis（PCOA）.Group I including 56 Southern grain sorghum germplasm resources is from guizhou,Sichuan and Yunnan mainly.GroupⅡincluding 48 Northern grain sorghum germplasm resources is from Liaoning,Inner Mongolia,shanxi,Beijing and Hebei mainly.GroupⅢincluding 52 Southern and Northern grain sorghum germplasm resources is from Heilongjiang,jilin,shaanxi,Shandong,Hubei and Yunnan.
出处
《贵州农业科学》
CAS
2016年第9期13-19,共7页
Guizhou Agricultural Sciences
基金
贵州省农业科学院课题[黔农科院院专项(2014)034]
贵州省农业科学院课题"黔高7号的示范与推广"[黔农科院院专项(2013)013]
贵州省科学技术基金项目"酱香型白酒用高粱种质资源遗传多样性的调查及关键功能基因的发掘"[黔科合J字LKN(2013)26]
贵州省科技计划课题"贵州省高粱工程技术研究中心"[黔科合农G字(2012)4004]
"酱香型白酒用糯高粱新品种的选育"[黔科合NY(2015)3021-2]
关键词
籽粒高粱
分子标记
遗传多样性
群体结构
grain sorghum
molecular marker
genetic diversity
population structure
