摘要
目的研究直肠腺癌组织Galectin-3、Fascin-1和ABI1的表达及其与临床病理参数的关系,以及三者之间的相关性。方法纳入60例直肠腺癌患者(直肠腺癌组)和60例健康体检者(健康对照组)作为研究对象,分别采集直肠腺癌和正常直肠黏膜组织,采用微波免疫组化技术检测直肠腺癌和正常直肠黏膜组织中Galectin-3、Fascin-1及ABI1表达情况,分析三种蛋白表达与临床病理的相关性,进一步分析三者之间的相关性。结果直肠腺癌组Galectin-3、Fascin-1及ABI1的表达水平显著高于正常对照组(P<0.05)。Galectin-3、Fascin-1及ABI1在不同临床分期、分化程度、浆膜浸润、淋巴结及远处转移直肠腺癌患者中的表达水平差异显著(P<0.05)。线性分析发现Galectin-3与Fascin-1和ABI1均呈正相关(r=0.9621、P=0.0000,r=0.9459、P=0.0000)。Fascin-1与ABI1呈正相关性(r=0.9633,P=0.0000)。结论 Galectin-3、Fascin-1及Ab I高表达均与直肠腺癌高临床分期、低分化程度、浆膜浸润、淋巴结转移有关,Galectin-3、Fascin-1及Ab I三者之间两两均呈正相关。
Objective To study the expression of Galectin-3, Fascin-1 and Ab1 interacting protein 1（ABI1） in rectal adenocarcinoma and its relation with clinicopathological features as well as the correlation among three proteins. Methods 60 patients with rectal adenocarcinoma（rectal adenocarcinoma group）and 60 healthy subjects（healthy control group） were selected as the subjects,the rectal adenocarcinoma and normal rectal mucosa were collected. The expression of Galectin-3, Fascin-1 and ABI1 in rectal adenocarcinoma and normal rectal mucosa were detected by microwave immunohistochemical technique, the correlation between the expression of three proteins and clinical pathology were analyzed. Results The high expression rate of Galectin-3, Fascin-1 and ABI1 in rectal adenocarcinoma group were significantly higher than that in normal control group（P 〈 0.05）.The high expression rates of Galectin-3,Fascin-1and ABI1 were significantly different in patients with different clinical stages, differentiation, serosa infiltration, lymph node and distant metastasis（P 〈 0.05）.The linear analysis showed that Galectin-3 were positively correlated with Fascin-1 and ABI1（r = 0.9621, P =0.0000; r = 0.9459, P = 0.000）, and Fascin-1 was positively correlated with ABI1（r = 0.9633, P = 0.0000）.Conclusion The high expression of Galectin-3, Fascin-1 and Ab I have correlate with the invasion and metastasis of rectal adenocarcinoma, and there were positive linear correlations among the three proteins.
作者
赵剑
戴敏
Zhao Jian Dai Min.(Department of Pathology, The Second People＇s Hospital of Wuhu, Wuhu, Anhui, 241000, China)
出处
《结直肠肛门外科》
2017年第4期435-439,共5页
Journal of Colorectal & Anal Surgery
