摘要
目的:研究热休克蛋白HDJ1在人肺腺癌组织、肺腺癌细胞株以及正常肺上皮细胞株中的表达情况,探讨HDJ1对肺腺癌细胞侵袭和迁移能力的影响。方法:采用免疫组织化学法检测HDJ1在人肺腺癌组织和癌旁正常组织中的表达情况;Western blot检测HDJ1在人肺腺癌细胞A549、H1299、H292及正常肺上皮细胞Bease-2B中的表达情况;使用p Magic 7.1-HDJ1-KD慢病毒转染A549细胞构建HDJ1稳定下调表达的细胞模型,并通过划痕实验、Transwell实验检测细胞的侵袭和迁移能力。结果:HDJ1在人肺腺癌组织中呈高表达;HDJ1在A549细胞中呈高表达;细胞划痕实验结果显示,与对照组细胞比较,HDJ1表达下调的A549细胞迁移率下降(P<0.05);Transwell实验结果表明,相对于对照组细胞,HDJI表达下调的A549细胞在侵袭和迁移实验中的穿膜细胞数均减少(P<0.05)。结论:HDJ1在肺腺癌组织和细胞中均呈高表达,并促进肺腺癌细胞的侵袭和迁移能力。
OBJECTIVE:To investigate expressions of HDJ1in lung adenocarcinoma tissues and cell lines,andto reveal the role of HDJ1in invasive ability of lung adenocarcinoma.METHODS:Immunohistochemistry method wasused to detect expressions of HDJ1in lung adenocarcinoma tissues and adjacent normal tissues.Western blot method wasapplied to determine expressions of HDJ1in human lung adenocarcinoma cell lines(A549,H1299,H292)and normallung epithelial cell line(Bease-2B).pMagic7.1-HDJ1-KD lentivirus was transfected into the A549cell line to construct aHDJ1down-expressed cell line,and pMagic7.1-NC lentivirus was transfected into the same cells as the control.Theinvasion ability of HDJ1down-expressed group and control group was analyzed using the Transwell and the WoundHealing assays.RESULTS:The expression levels of HDJ1in lung adenocarcinoma tissues were significantly higher thanthose in the adjacent normal tissues.HDJ1was over-expressed in the A549cell line.Compared with the control group,the Wound Healing assay results show that,the migration and healing ability of the HDJ1down-expressed A549cellswere decreased.At48h after transfection,both the invasion and migration abilities of the HDJ1down-expressed A549cells were lower than that of control cells(P<0.05).CONCLUSION:Heat shock protein HDJ1were highly expressed inthe lung adenocarcinoma tissues and cell lines.In addition,HDJ1promoted the invasion and migration ability of lungadenocarcinoma cells.
作者
李雨轩
李凯
张彤彤
郭元彪
殷俊
包勇
LI Yuxuan;LI Kai;ZHANG Tongtong;GUO Yuanbiao;YIN Jun;BAO Yong(Respiratory Medicine of the Third People's Hospital of Chengdu, Chengdu 610031, Sichuan, China)
出处
《癌变．畸变．突变》
CAS
CSCD
2017年第5期358-363,共6页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
四川省应用基础研究计划项目(2014JY0136)
四川省科技厅基金(2014SZ0055)
关键词
热休克蛋白
HDJ1
肺腺癌
侵袭
迁移
heat shock protein
HDJ1
lung adenocarcinoma
invasion
migration
