摘要
【目的】在前期研究中,本实验室已克隆了一个水稻小热休克蛋白基因(OsSHSP17.6),并发现该基因的表达明显受到热激和病毒侵染调控,表明该蛋白可能在逆境胁迫过程中起重要作用。本研究的目的在于进一步明确OsSHSP17.6的特性。【方法】在本研究中,进一步将该基因亚克隆至原核表达载体p ET-32a并导入大肠杆菌E.coli BL21(DE3)pLysS诱导表达,通过亲和层析的方法纯化了该重组蛋白,进一步用于非变性聚丙烯酰胺凝胶电泳和Western blotting分析。【结果】异源表达的重组OsSHSP17.6能减轻IPTG对宿主菌的毒害。非变性聚丙烯酰胺凝胶电泳和Western blotting分析显示纯化的重组OsSHSP17.6蛋白在体外能形成同源二聚体和寡聚体。【结论】这些结果支持OsSHSP17.6是一个有功能的分子伴侣蛋白并表明该蛋白可能通过形成同源寡聚体的方式参与逆境胁迫反应,这为进一步明确OsSHSP17.6的功能机制奠定基础。
【Objective】In our previous study,a small heat shock protein gene OsSHSP17.6was cloned from Oryza sativaand its expression was shown to be significantly up-regulated by heat shock or viral infection,suggesting that theOsSHSP17.6could play an important role in both biotic and abiotic stress responses.In this study,our objective is to furtheridentify the characteristics of OsSHSP17.6.【Method】The OsSHSP17.6gene was sub-cloned into the plasmid pET-32a,a prokaryotic expression vector,and transformed into Escherichia coli BL21(DE3)pLysS for inducible expression.Thenthe recombinant protein was purified with affinity chromatography and used for native PAGE and Western-blotting assays.【Result】Its heterologous expression appeared to alleviate the poisonous effect of IPTG on the host E.coli.Native PAGEand Western-blotting assays showed that the purified recombinant OsSHSP17.6could form homological dimers andoligomer in vitro.【Conclusion】Taken together,these findings supported the hypothesis that the protein should befunctional molecular chaperone in vivo and indicated that OsSHPS17.6could be involved in the stress response by itshomological oligomerization,which could contribute to the functional identification of OsSHSP17.6.
作者
蔡年俊
郭留明
李静
项聪英
羊健
陈剑平
张恒木
CAI Nianjun;GUO Liuming;LI Jing;XIANG Congying;YANG Jian;CHEN Jianping;CHEN Jianping
出处
《中国水稻科学》
CAS
CSCD
北大核心
2017年第5期483-488,共6页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(31601603)
浙江省自然科学基金资助项目(LQ14C140003)
关键词
小热休克蛋白
异源表达
寡聚化作用
small heat shock protein (SHSP)
heterologous expression
oligomerization
