摘要
目的:探索Th17细胞及IL-17、IL-21在牛奶蛋白过敏(CMA)婴儿发病机制中的作用。方法:采集病例组治疗前后和健康对照组各25例患者的空腹外周静脉血,用流式细胞仪技术测定Th17细胞在CD4^+T细胞中的占比,用ELISA法检测上清IL-17、IL-21水平,比较两组的各项指标。结果:病例组治疗前Th17细胞占比(8.97±2.81)%,治疗后(3.72±2.52)%,对照组(1.92±1.38)%,比较差异均有统计学意义(P<0.05)。IL-17在对照组、病例组治疗前、病例组治疗后分别为(3.05±1.61)、(25.58±7.89)、(13.07±9.63)ng/m L,IL-21在对照组、病例组治疗前、病例组治疗后分别为(1.88±1.62)、(13.43±4.3)、(9.13±4.67)ng/m L,比较差异均有统计学意义(P<0.05)。结论:Th17细胞、IL-17和IL-21可能为CMA的研究及治疗提供了靶向依据。
Objective：To explore the role of Th17 cells,IL-17 and IL-21 in the pathogenesis of cow’s milk allergy（CMA）.Method：The fasting peripheral venous blood in control group of 25 cases and before and after treatment in case group of 25 cases were collected,the percentage of Th17 cells in CD4＋ T cells was measured by flow cytometry,the levels of IL-17 and IL-21 in supernatant were detected by enzyme-linked immunosorbent assay（ELISA）,those indicators of two groups were compared.Result：The proportion of Th17 cells was （8.97±2.81）% before treatment,（3.72±2.52）% after treatment and （1.92±1.38）% in the control group,the differences were statistically significant（P〈0.05）.In the control group,the case group before treatment and after treatment IL-17 were respectively （3.05±1.61）,（25.58±7.89）and （13.07±9.63）ng/mL;IL-21 were respectively （1.88±1.62）,（13.43±4.3） and （9.13±4.67）ng/mL, compared of those,the differences were statistically significant（P〈0.05）.Conclusion： Th17 cells,IL-17 and IL-21 may provide potential targets for the study and therapy of CMA.
出处
《中国医学创新》
CAS
2017年第28期44-47,共4页
Medical Innovation of China
