摘要
为了进一步理解己糖转运蛋白基因家族的结构特征以及其在植物逆境信号转导过程中的作用,基于小桐子基因组数据,鉴定到15个小桐子己糖转运蛋白基因,并对其基因结构、进化关系、密码子偏性及低温表达特性进行了分析。结果表明,15个小桐子HXT基因聚类为5个亚组,包含2~5个外显子,且在基因上游调控区包含ABA、乙烯、干旱及低温等逆境响应元件。编码蛋白质都富含疏水性氨基酸,具有典型的12个α-螺旋跨膜区。另外,部分小桐子HXT基因存在交叉共线性关系及基因倍增现象,且密码子的第3位碱基偏好使用U或A。荧光定量分析显示,Jc HXT_13L与Jc HXT_C基因在小桐子的各器官中都有表达,其中,Jc HXT_13L在茎中表达量较高,而Jc HXT_C在根中表达量较高,Jc HXT_13L与Jc HXT_C在根与叶片中受低温诱导上调表达明显,与小桐子的抗冷性形成直接相关。为进一步探索小桐子HXT基因的生物学功能及参与小桐子抗冷分子机制奠定了理论基础。
In order to further understand the structure characteristic of hexose transporter and its functions in plant stress signal transduction,15 HXT genes were identified based on complete genome data of Jatropha curcas,then the gene structure,evolutionary relationship,codon bias,and chilling expression feature were analyzed. The results showed that 15 J. curcas HXT genes were classified to 5 subgroups,and owned 2-5 exons,cis-acting elements response to ABA,ethylene,drought and cold were found in the gene upstream. Encoding proteins with abundant hydrophobic amino acids possess typical 12 α-helix transmembrane regions. Gene location analysis revealed that some HXT genes were observed the phenomenons of cross-collinearity and gene paleopolyploidy. Codon bias analysis showed that HXT genes in J. curcas were biased toward the synonymous codons with U or A at the third codon position. RT-q PCR analysis revealed that Jc HXT_13 L and Jc HXT_C expressed differently in different tissues,with abundant in stem and root,and remarkably cold-induced expression in root and leaf,respectively. This study laid a theoretical foundation for further dissection on biological function and chilling-resistance molecular mechanism of HXT family in J. curcas.
作者
王海波
高永
辛胡
WANG Haibo GAO Yong XIN Hu(Qujing Normal University, Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing 655011, China Qujing Normal University, Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on YunGui Plateau,Qujing 655011 ,China)
出处
《华北农学报》
CSCD
北大核心
2017年第5期97-105,共9页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31460179)
关键词
小桐子
己糖转运蛋白
己糖信号
基因组
表达分析
Jatropha curcas
Hexose transporter
Hexose signal
Genome
Expression analysis
