摘要
This study aimed to solve the issues in safe wintering of alfalfa in the north of China. The^(60)Co-γ rays, ultraviolet and EMS were used to mutagenize seeds of 4 cultivars of alfalfa to investigate the effects of mutagens on antioxidant enzymes activity and cold resistance of alfalfa. The results showed that after the mutagenic treatment, the activity of the three kinds of antioxidant enzymes basically showed upward trends.^(60)Co-γ radiation increased the activity of SOD; ultraviolet radiation decreased the activity of SOD; and low-concentration EMS promoted and high-concentration EMS inhibited the activity of SOD. The activity of POD and CAT decreased with the increased radiation dose of^(60)Co-γ, but increased with the increased radiation dose of ultraviolet. The effects of EMS treatment on the activity of POD and CAT differed among different alfalfa cultivars. The concentration of MDA reduced under 30-min, 60-min ultraviolet treatment and 0.4% EMS treatment. According to the subordinate function values of various indices, it could be concluded that 150 Gy of^(60)Co-γ radiation, 90 min of ultraviolet radiation, and 0.4% of EMS were more conducive to improving the cold resistance of alfalfa. This study will provide a theoretical basis for the research on adaptability and cold resistance of alfalfa in rigid cold region and a technical reference for the breeding of high-yield, highquality and cold-resistant alfalfa cultivars.
This study aimed to solve the issues in safe wintering of alfalfa in the north of China. The60Co-γ rays, ultraviolet and EMS were used to mutagenize seeds of 4 cultivars of alfalfa to investigate the effects of mutagens on antioxidant enzymes activity and cold resistance of alfalfa. The results showed that after the mutagenic treatment, the activity of the three kinds of antioxidant enzymes basically showed upward trends.60Co-γ radiation increased the activity of SOD; ultraviolet radiation decreased the activity of SOD; and low-concentration EMS promoted and high-concentration EMS inhibited the activity of SOD. The activity of POD and CAT decreased with the increased radiation dose of60Co-γ, but increased with the increased radiation dose of ultraviolet. The effects of EMS treatment on the activity of POD and CAT differed among different alfalfa cultivars. The concentration of MDA reduced under 30-min, 60-min ultraviolet treatment and 0.4% EMS treatment. According to the subordinate function values of various indices, it could be concluded that 150 Gy of60Co-γ radiation, 90 min of ultraviolet radiation, and 0.4% of EMS were more conducive to improving the cold resistance of alfalfa. This study will provide a theoretical basis for the research on adaptability and cold resistance of alfalfa in rigid cold region and a technical reference for the breeding of high-yield, highquality and cold-resistant alfalfa cultivars.
