摘要
用浓度为0.1mmol/L外源NO供体硝普钠(sodium nitroprusside,SNP)处理4℃吸胀冷害下紫花苜蓿种子,来探讨吸胀冷害下NO对紫花苜蓿种子萌发及氧化损伤的影响。试验设计4个处理,分别为A:对照组(CK)为蒸馏水;B:4℃吸胀冷害组(Chilling);C:SNP;D:Chilling+SNP,每个处理重复3次。结果表明:1)紫花苜蓿种子经SNP处理12h转入25℃继续培养84h后,Chilling+SNP组比Chilling组发芽率、活力指数、发芽指数提高了14.37%,34.17%,1.29%。2)4℃吸胀冷害12h后,Chilling+SNP组同Chilling组相比超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性分别提高了149.26%,60.35%,过氧化氢(H2O2)含量及超氧阴离子(·O2-)产生速率则降低了10.11%,27.61%。3)SNP对4℃吸胀冷害12,24和48h的种子体内羟自由基(·OH)的清除均呈下降趋势,Chilling+SNP组的清除率均高出SNP组,Chilling+SNP组在冷害12h时清除率达最大值97.5%。综上所述,施加外源NO能促进种子的萌发,提高抗氧化酶活性,降低了活性氧对种子造成的伤害。
Seeds of Medicago sativa were used to study the effects of 0.1 mmol/L sodium nitroprusside （SNP）, as a source of nitric oxide,on seed germination and antioxidant capacity under imbibitional chilling at 4℃.Four treatments：control,chilling,SNP and chilling＋SNP were replicated three times.The results indicated that after imbibitional chilling for 12 h and germination at 25℃ for 84 h,the vigor and germination of M.sativa seeds were increased by SNP treatment.The activities of antioxidant enzymes including superoxide dismutase （SOD）and catalase （CAT）increased 149.3% to 60.4% by SNP treatment.The radical clearance rate tended to decline with SNP treatments and chilling for 12,24 and 48 h.At 12 h chilling the radical clearance rate reached a maximum of 97.5%.Imposing exogenous nitric oxide can promote germination,enhance antioxidant enzyme activities and reduce the reactive oxygen damage in M.sativa seed.
出处
《草业学报》
CSCD
北大核心
2015年第4期87-94,共8页
Acta Prataculturae Sinica
基金
甘肃省自然基金(145RJZA196)资助
关键词
一氧化氮
紫花苜蓿
吸胀冷害
抗氧化性
nitric oxide
Medicago sativa seed
chilling imbibition
antioxidant
