摘要
根据甘蓝测序结果,利用CodonW软件对结球甘蓝的48 000个蛋白质编码基因序列进行了分析,计算出同义密码子相对使用频率,确定了TCT、CCT、AGA、GTT、GCT、GAT等6个高频密码子。将甘蓝与大肠杆菌、拟南芥、棉花、水稻的密码子使用频率进行了比较,发现甘蓝密码子使用偏爱性与同为双子叶植物的拟南芥、棉花基本一致,而与大肠杆菌及单子叶植物水稻均具有较大差异。以甘蓝密码子用法分析结果为依据,对Bt cry1C抗虫基因进行了密码子的改造,得到了具有甘蓝密码子使用特点的cry1C基因序列,为甘蓝的转基因研究奠定了分子基础。
Forty-eight thousand coding DNA sequences of cabbage were used to analyze the relative frequency of synonymous codon （RFSC）. Six high-frequency codons including TCT, CCT, AGA, GTT, GCT, and GAT were revealed by CodonW. When the frequency of codon usage of cabbage was compared to Escherichia coli, Arabidopsis thaliana, and Oryza sativa, we found that the codon preference was considerably different between cabbage and Escherichia coli, Oryza sativa, while the codon preference was identical between cabbage and Arabidopsis thaliana, Gossypium hirsutum. The codons of Bt crylC gene sequence have been reconstructed according to the results. The foreign gene containing the optimal codons of cabbage is helpful to researches involving cabbage transformation.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第10期1927-1934,共8页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31071697)
国家‘863’计划项目(2008AA10Z105)
国家大宗蔬菜产业技术体系项目(nycytx-35-gw01)
‘十二五’国家科技支撑计划项目(2012BAD02B01)
农业部园艺作物生物学与种质创制重点实验室项目
