摘要
目的研究原发性干燥综合征(primary sjogren's syndrome,PSS)患者外周血OX40、OX40L、PD-1、PD-L1和SPD-1的表达水平及临床意义。方法选择本院2012年3月—2015年3月收治的50例PSS患者(观察组),再根据疾病活动情况及病情程度分为活动期亚组(30例)、非活动期亚组(20例)和单系统亚组(29例)、多系统亚组(21例)。选择同期我院行体检健康者50例作为对照组。两组均行免疫荧光标记、流式细胞术检测外周静脉血OX40、OX40L、PD-1、PD-L1和SPD-1的表达情况;比较所有纳入研究者间OX40、OX40L、PD-1、PD-L1及SPD-1的表达情况,并记录观察组患者治疗前后上述指标的表达情况。结果观察组CD4+OX40+、CD14+OX40L+、CD19+OX40L+、CD4+PD-1+和CD4+PD-1+及SPD-1表达均高于对照组,CD14+PD-L1+表达低于对照组,差异均有统计学意义(P<0.01)。对观察组亚组进行分析,活动期亚组CD4+OX40+、CD14+OX40L+、CD19+OX40L+、CD4+PD-1+及SPD-1表达显著高于非活动期亚组(P<0.05或P<0.01);单系统亚组CD4+OX40+、CD14+OX40L+、CD19+OX40L+及SPD-1表达明显低于多系统亚组(P<0.05或P<0.01)。治疗前观察组CD4+OX40+、CD14+OX40L+、CD19+OX40L+和CD4+PD-1+表达显著高于治疗后(P<0.05);CD8+PD-1+、CD14+PD-L1+及SPD-1表达治疗前后比较差异无统计学意义(P>0.05)。结论 PSS患者外周静脉血中OX40、OX40L、PD-1、PD-L1和SPD-1的异常表达,和疾病活动情况、组织损害情况有关联,可作为了解病情活动度和严重程度的检测指标。
Objective To investigate expressions and clinical significances of OX40, OX40L, PD-1, PD-L1 and SPD-1 in peripheral blood of patients with primary Sjogren＆#39;s syndrome （ PSS） . Methods A total of 50 patients with PSS （ ob-servation group） admitted during March 2012 and March 2015 were divided into active period subgroup （n=30） and inactivi-ty period subgroup （n=20） according to disease activity conditions, and single system subgroup （n=29） and multisystemic subgroup （n=21） according to active stages. Other 50 healthy people taking medical examinations at the same period were selected as control group. OX40, OX40L, PD-1, PD-L1 and SPD-1 expressions in peripheral venous blood were detected by immunofluorescence labeling and flow cytometry methods in two groups. OX40, OX40L, PD-1, PD-L1 and SPD-1 expressions were compared in groups and subgroups, and changes of the above indexes before and after treatment were recorded in observa-tion group. Results In observation group, CD4 ＋OX40 ＋, CD14 ＋OX40L＋, CD19 ＋OX40L＋, CD4 ＋PD-1 ＋ and CD4 ＋PD-1L＋ and SPD-1 expressions were significantly higher, while CD14 ＋PD-L1 ＋ was significantly lower than those in control group （P〈0. 01）. CD4 ＋OX40 ＋, CD14 ＋OX40L＋, CD19 ＋OX40L＋, CD4 ＋PD-1 ＋ and SPD-1 expressions in active period sub-group were significantly higher than those in inactivity period subgroup （ P 〈0. 05 or P 〈0. 01 ）; CD4 ＋OX40 ＋, CD14 ＋OX40L＋, CD19 ＋OX40L＋ and SPD-1 expressions in single system subgroup were significantly lower than those in multisys-temic subgroup （ P〈0. 05 or P〈0. 01 ） . In 10 preliminary diagnosis patients in observation group, CD4 ＋OX40 ＋, CD14 ＋OX40L＋, CD19 ＋OX40L＋ and CD4 ＋PD-1 ＋ expressions before treatment were significantly higher than those after treatment （ P〈0. 05）;there were no significant differences in CD8 ＋PD-1 ＋, CD14 ＋PD-L1 ＋ and SPD-1 expression between before and after treatment （P〉0. 05）. Conclusion A
出处
《临床误诊误治》
2017年第10期83-87,共5页
Clinical Misdiagnosis & Mistherapy
基金
四川省卫生厅科研课题资助(120003)
