摘要
目的:观察人参皂苷对波动性高糖大鼠氧化应激水平及单核细胞趋化因子-1(MCP-1)和肿瘤坏死因子-α(TNF-α)在大鼠肾脏及主动脉组织表达的影响。方法:将48只雄性SD大鼠随机分成正常对照组8只(N组)和糖尿病模型组40只。用高脂饲料喂养模型组大鼠4周,予小剂量链脲佐菌素(STZ)诱导建立糖尿病大鼠模型,将其随机分为稳定高糖组(S组)8只和波动性高糖模型组32只,错时注射葡萄糖、胰岛素制备血糖波动模型。2周后,将波动性高糖模型组随机分为人参皂苷低(FL组)、中(FM组)、高剂量组(FH组)及波动性高糖组(FO组),各8只,人参皂苷低、中、高各剂量组予相应剂量[14 mg(kg·d)、28 mg/(kg·d)、56 mg/(kg·d)]的人参皂苷灌胃8周,波动性高糖组(FO组)给予等量的生理盐水灌胃。测定大鼠血清活性氧(ROS)浓度,取肾脏及主动脉组织,观察其病理变化,并测定MCP-1和TNF-α在大鼠肾脏及主动脉组织的表达情况。结果:与正常对照组(N组)比较,稳定高糖组(S组)及波动性高糖组(FO组)各时间点血糖水平明显增高;血清ROS浓度均明显增加;血管和肾脏组织MCP-1和TNF-α的表达均增多(P<0.05)。与稳定高糖组(S组)比较,波动性高糖组(FO组)各时间点的血糖水平变化较大(P<0.05),其在1天内有2个明显的血糖波动高峰和低谷;血清ROS浓度明显增加;血管和肾脏组织MCP-1和TNF-α表达增多(P<0.05)。与波动性高糖组(FO组)比较,人参皂苷低(FL组)、中(FM组)、高剂量组(FH组)血清ROS浓度均明显下降,血管和肾脏组织组MCP-1和TNF-α表达均明显下降(P<0.05)。人参皂苷低剂量组(FL组)、中剂量组(FM组)、高剂量组(FH组)组间比较,人参皂苷浓度越高,血清ROS浓度、MCP-1和TNF-α的表达越低(P<0.05)。结论:人参皂苷可以下调氧化应激,抑制MCP-1和TNF-α的过表达对血管组织的损伤,对波动性高糖所致血管病变有一定的保护作用。
Objective： To observe the effect of Ginsenoside on oxidative stress level and expression of monocyte chemoattractant protein-1（MCP-1） and tumor necrosis factor-α（TNF-α） in renal and aortic tissue of rat with Fluctuating high glucose. Methods：Divided 48 SD male rats into the normal control group（Ngroup） being 8 cases and the diabetes model group being 40 cases randomly. The diabetes model group was fed with high-fat diet for four weeks, and was given streptozocin（STZ） of lowdose so as to establish the diabetes models; divided the diabetes models into the stabled high glucose group（S group） being 8 cases,and the fluctuating high glucose group being 32 cases which were given interleaved injection of glucose and insulin to make the fluctuating glucose models. After two weeks,divided the fluctuating high glucose group into the lowdose of G insenoside group（FL group）, moderate dose of Ginsenoside group（FM group）, high dose of G insenoside group（FH group） and the fluctuating high glucose group（FOgroup）,8 cases in each group. The FL group,FMgroup and FHgroup were given G insenoside of 14 mg/（kg·d）,28 mg/（kg·d） and 56 mg/（kg·d） respectively for eight weeks,while the FOgroup was given saline of equivalent volume by gavage. D etected concentration of serumreactive oxygen species（ROS） in rats,and observed pathological changes of renal and aortic tissue of rats,detected expression of MCP-1and TNF-α in renal and aortic tissue. Results： Compared with the Ngroup, blood glucose levels of every time point,concentration of serumROS, expression of MCP-1 and TNF-αin blood vessel and renal tissue were all significantly increased in the S group and the FOgroup（P0.05）. Compared with the S group,change of blood glucose levels of every time point in the FOgroup was more obviously（P0.05）,which performed by two obvious peak and lowebb of glucose variability,significantly increased concentration of serum ROS and expression of MCP-1 and TNF-α in blood vessel and renal t
出处
《新中医》
CAS
2017年第10期12-15,共4页
New Chinese Medicine
基金
浙江省中医药管理局项目(2014ZA045)
