摘要
目的:探讨表没食子儿茶素没食子酸酯(EGCG)和维生素E(Vit E)对铅暴露仔鼠学习记忆能力的影响。方法:将健康SPF级昆明雌鼠随机分为对照组(4只)和染毒组(20只)。染毒组饮2 g/L醋酸铅溶液染毒21 d,对照组饮无铅去离子水21 d。染毒结束后按雌雄比2∶1合笼饲养。分娩后第21天,将染毒组所产仔鼠随机分为7组,即生理盐水组、玉米油组、EGCG干预组(3.0 mg/kg)、VitE干预组(30 IU/kg)、联合干预1组(EGCG 3.0 mg/kg+VitE 30 IU/kg)、联合干预2组(EGCG 1.5 mg/kg+VitE 60 IU/kg)、联合干预3组(EGCG 6.0 mg/kg+VitE 15IU/kg),每组10只;随机选取10只对照组雌鼠所产仔鼠作为正常对照组。正常对照组仔鼠饮无铅去离子水,其余7组仔鼠灌胃相应试剂,共干预28 d。灌胃结束后,用Morris水迷宫测定小鼠学习记忆能力,用极谱仪测定小鼠血铅含量,用氧化酶试剂盒测定海马组织中SOD活性和MDA含量。结果:与正常对照组相比,生理盐水组和玉米油组逃避潜伏期延长,穿越平台次数减少,血铅含量升高,海马组织中SOD活性降低,MDA含量增加(P<0.05)。与生理盐水组和玉米油组相比,各干预组逃避潜伏期缩短,穿越平台次数增多,血铅含量不同程度降低,联合干预1、3组SOD活性升高,MDA含量降低(P<0.05)。结论:EGCG和VitE可能通过提高海马组织中SOD活性,改善仔鼠的学习记忆能力,对铅暴露仔鼠神经损伤有一定的保护作用。
Aim： To investigate the effects of epigallocatechin gallate（ EGCG） combined with vitamin E（ Vit E） on learning and memory ability and antioxidative damage in hippocampus of progestational lead-exposed newborn mice. Methods： A total of 20 healthy mice were exposed to 2 g/L lead acetate aqueous solution,and 4 mice were given lead-free deionized water for 21 days. After that,the female and male mice were fed in one cage with 2∶1 ratio. At the 21 th day after delivery,the offspring of lead-exposed female mice were divided into 7 groups randomly： physiological saline group,corn oil group,EGCG group（ 3. 0 mg/kg）,Vit E group（ 30 IU/kg）,combination group Ⅰ（ EGCG 3. 0 mg/kg ＋ Vit E 30 IU/kg）,combination group Ⅱ（ EGCG 1. 5 mg/kg ＋ Vit E 60 IU/kg）,combination group Ⅲ（ EGCG 6. 0 mg/kg ＋ Vit E 15 IU/kg）,and received corresponding intervention for 28 days,10 in each group; the offspring（ n = 10） of lead-free female mice were the normal control,and received lead-free deionized water for 28 days. After the intervention,the learning and memory ability of mice was measured by Morris water maze,the blood lead level was measured by voltammetry,and the activities of SOD and the concentration of MDA in hippocampus were measured by Oxidase Kit. Results： Compared with the normal control group,the escape latency of the physiological saline group and the corn oil group increased,the times across the platform decreased,the blood lead level and MDA concentration increased,and SOD activity decreased（ P〈0. 05）. Compared with the physiological saline group and the corn oil group,the escape latency of the 5 intervention groups dcreased,the times across the platform increased,the blood lead level reduced in different degree（ P〈0. 05）; the SOD activity of combination group Ⅰ and Ⅲ increased,and MDA concentration decreased（ P〈0. 05）. Conclusion： EGCG and Vit E might improve the learning and memory ability of lead-exposed offspring mice through increasing the SOD activity
出处
《郑州大学学报（医学版）》
CAS
北大核心
2017年第5期574-578,共5页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目81172716
关键词
EGCG
维生素E
孕前期铅暴露
小鼠
学习记忆能力
抗氧化损伤
epigallocatechin gallate
vitamin E
lead exposed during progestational
mouse
learning and memory ability
antioxidative injury
